|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
2007-4-9
|
|
pubmed:abstractText |
We aimed to optimize non-viral transfection of human stromal cell derived factor (SDF-1alpha) gene into skeletal myoblasts (SkM) and, transplant these cells to establish transient SDF-1alpha gradient to favor extra-cardiac stem cell translocation into infarcted heart. Optimized conditions for transfection of SDF-1alpha gene into syngenic SkM were achieved using FuGene6/phSDF-1alpha (3:2v/w, 4 h transfection) with 125 microM ZnCl(2) (p<0.001). After characterization for transgene overexpression by immunostaining, ELISA and PCR, the cells were transplanted in female rat model of myocardial infarction. Thirty-six rats were grouped (n=12/group) to receive 70 microl DMEM without cells (group-1) or containing 1.5 x 10(6) non-transfected (group-2) or SDF-1alpha transfected SkM (group-3). On day 4 post-transplantation (in 4 animals/group), marked expression of SDF-1alpha/sry-gene (p=0.003), total Akt, phospho-Akt and Bcl2 was observed in group-3. The number of CD31(+), C-kit(+) and CD34(+) cells was highest in group-3 hearts (p<0.01). Blood vessel density in group-3 was higher in both scar and peri-scar regions (p<0.001) as compared with other groups. Echocardiography showed improved indices of left ventricle contractile function and remodeling in group-3 (p<0.05) as compared with groups-1 and -2. We conclude that ex vivo SDF-1alpha transgene delivery promotes stem and progenitor cell migration to the heart, activates cell survival signaling and enhances angiomyogenesis in the infarcted heart.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-10406801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-10933955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-10974345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11413075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11437538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11489932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11504914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11568057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11756151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-11820456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12068293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12091877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12221648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12628955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12649349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-12957092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-14672915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15052220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15145120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15148275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15284120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15386736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15533866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15582707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15951423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-15979058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-16153908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-16413490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-16603183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-16690882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-16926022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-8941636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-9722546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17350033-9933168
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0022-2828
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
42
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
792-803
|
|
pubmed:dateRevised |
2011-4-25
|
|
pubmed:meshHeading |
pubmed-meshheading:17350033-Animals,
pubmed-meshheading:17350033-Cells, Cultured,
pubmed-meshheading:17350033-Chemokine CXCL12,
pubmed-meshheading:17350033-Chemokines, CXC,
pubmed-meshheading:17350033-Female,
pubmed-meshheading:17350033-Gene Therapy,
pubmed-meshheading:17350033-Hematopoietic Stem Cell Mobilization,
pubmed-meshheading:17350033-Hematopoietic Stem Cells,
pubmed-meshheading:17350033-Humans,
pubmed-meshheading:17350033-Male,
pubmed-meshheading:17350033-Muscle, Skeletal,
pubmed-meshheading:17350033-Myocardial Infarction,
pubmed-meshheading:17350033-Neovascularization, Physiologic,
pubmed-meshheading:17350033-Rats,
pubmed-meshheading:17350033-Rats, Inbred F344,
pubmed-meshheading:17350033-Rats, Wistar,
pubmed-meshheading:17350033-Stem Cell Transplantation,
pubmed-meshheading:17350033-Stromal Cells
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Ex vivo delivered stromal cell-derived factor-1alpha promotes stem cell homing and induces angiomyogenesis in the infarcted myocardium.
|
|
pubmed:affiliation |
Department of Pathology and Laboratory Medicine, 231-Albert Sabin Way, University of Cincinnati, Cincinnati, OH 45267-0529, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
