Linked Life Data

17261589

Source:http://linkedlifedata.com/resource/pubmed/id/17261589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2007-4-9
pubmed:abstractText
Atp6p is an essential subunit of the ATP synthase proton translocating domain, which is encoded by the mitochondrial DNA (mtDNA) in yeast. We have replaced the coding sequence of Atp6p gene with the non-respiratory genetic marker ARG8m. Due to the presence of ARG8m, accumulation of rho-/rho0 petites issued from large deletions in mtDNA could be restricted to 20-30% by growing the atp6 mutant in media lacking arginine. This moderate mtDNA instability created favorable conditions to investigate the consequences of a specific lack in Atp6p. Interestingly, in addition to the expected loss of ATP synthase activity, the cytochrome c oxidase respiratory enzyme steady-state level was found to be extremely low (<5%) in the atp6 mutant. We show that the cytochrome c oxidase-poor accumulation was caused by a failure in the synthesis of one of its mtDNA-encoded subunits, Cox1p, indicating that, in yeast mitochondria, Cox1p synthesis is a key target for cytochrome c oxidase abundance regulation in relation to the ATP synthase activity. We provide direct evidence showing that in the absence of Atp6p the remaining subunits of the ATP synthase can still assemble. Mitochondrial cristae were detected in the atp6 mutant, showing that neither Atp6p nor the ATP synthase activity is critical for their formation. However, the atp6 mutant exhibited unusual mitochondrial structure and distribution anomalies, presumably caused by a strong delay in inner membrane fusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10853-64
pubmed:meshHeading
pubmed-meshheading:17261589-Adenosine Triphosphate, pubmed-meshheading:17261589-Cell Shape, pubmed-meshheading:17261589-Cytochromes c, pubmed-meshheading:17261589-DNA, Mitochondrial, pubmed-meshheading:17261589-Gene Deletion, pubmed-meshheading:17261589-Gene Expression Regulation, Fungal, pubmed-meshheading:17261589-Genes, Mitochondrial, pubmed-meshheading:17261589-Genotype, pubmed-meshheading:17261589-Hydrolysis, pubmed-meshheading:17261589-Microscopy, Electron, pubmed-meshheading:17261589-Mitochondria, pubmed-meshheading:17261589-Mitochondrial Proton-Translocating ATPases, pubmed-meshheading:17261589-Oxidoreductases, pubmed-meshheading:17261589-Oxygen, pubmed-meshheading:17261589-Protein Biosynthesis, pubmed-meshheading:17261589-Saccharomyces cerevisiae, pubmed-meshheading:17261589-Transaminases, pubmed-meshheading:17261589-Transcription, Genetic
pubmed:year
2007
pubmed:articleTitle
Yeast cells lacking the mitochondrial gene encoding the ATP synthase subunit 6 exhibit a selective loss of complex IV and unusual mitochondrial morphology.
pubmed:affiliation
Institut de Biochimie et Génétique Cellulaires, Université Victor Segalen, 1 Rue Camille Saint-Saëns, 33077 Bordeaux cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't