Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1991-11-25
|
| pubmed:databankReference | |
| pubmed:abstractText |
Chlorella sorokiniana possesses ammonium-inducible, chloroplastic, NADP-specific glutamate dehydrogenase (NADP-GDH) homo- or heterohexamers composed of alpha- and/or beta-subunits which were previously shown to derive from precursor protein(s) of identical size. From the present studies, data are consistent with these two subunits being encoded by a single nuclear gene. The NADP-GDH gene is greater than 7 kb in length due to the presence of at least 21 introns, an unusually large number for a eukaryotic microorganism. The exons, identified by comparison with sequences of NADP-GDH cDNA clones, include a region which is highly conserved among NADP-GDH genes. This region in the C. sorokiniana gene is 77% and 73% identical to the corresponding regions in the Escherichia coli and Neurospora crassa NADP-GDH genes, respectively. Seventeen independent NADP-GDH cDNA clones were analyzed by restriction mapping and partial sequencing, and no differences were detected among them. The longest cDNA was fused in frame with lacZ in a Bluescript vector and was expressed in E. coli as NADP-GDH antigen. During a 240 min induction period, under conditions in which both types of subunits were synthesized, only a single (2.2 kb) NADP-GDH mRNA band was detected on northern blots using cDNA probes from the highly conserved and 3'-untranslated regions. Collectively, these results are consistent with a single mRNA encoding a precursor-protein which is differentially processed to yield either an alpha- or beta-subunit.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ammonia,
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0167-4412
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1023-44
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1718478-Amino Acid Sequence,
pubmed-meshheading:1718478-Ammonia,
pubmed-meshheading:1718478-Base Sequence,
pubmed-meshheading:1718478-Blotting, Northern,
pubmed-meshheading:1718478-Blotting, Southern,
pubmed-meshheading:1718478-Cell Nucleus,
pubmed-meshheading:1718478-Chlorella,
pubmed-meshheading:1718478-Chloroplasts,
pubmed-meshheading:1718478-Cloning, Molecular,
pubmed-meshheading:1718478-Codon,
pubmed-meshheading:1718478-DNA,
pubmed-meshheading:1718478-Enzyme Induction,
pubmed-meshheading:1718478-Escherichia coli,
pubmed-meshheading:1718478-Gene Library,
pubmed-meshheading:1718478-Genes,
pubmed-meshheading:1718478-Glutamate Dehydrogenase,
pubmed-meshheading:1718478-Introns,
pubmed-meshheading:1718478-Macromolecular Substances,
pubmed-meshheading:1718478-Molecular Sequence Data,
pubmed-meshheading:1718478-Neurospora crassa,
pubmed-meshheading:1718478-RNA,
pubmed-meshheading:1718478-RNA, Messenger,
pubmed-meshheading:1718478-Restriction Mapping,
pubmed-meshheading:1718478-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana.
|
| pubmed:affiliation |
Department of Microbiology and Cell Science, University of Florida 32611.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|