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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1991-10-4
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pubmed:databankReference |
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pubmed:abstractText |
The tsx-p2 promoter is one of at least seven Escherichia coli promoters that are activated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex and negatively regulated by the CytR repressor. DNase I footprinting assays were used to study the interactions of these regulatory proteins with the tsx-p2 promoter region and to characterize tsx-p2 regulatory mutants exhibiting an altered response to CytR. We show that the cAMP-CRP activator complex recognizes two sites in tsx-p2 that are separated by 33 bp: a high-affinity site (CRP-1) overlaps the -35 region, and a low-affinity site (CRP-2) is centered around position -74 bp. The CytR repressor protects a DNA segment that is located between the two CRP sites and partially overlaps the CRP-1 target. In combination, the cAMP-CRP and CytR proteins bind cooperatively to tsx-p2, and the nucleoprotein complex formed covers a region of 78 bp extending from the CRP-2 site close to the -10 region. The inducer for the CytR repressor, cytidine, does not prevent in vitro DNA binding of CytR, but releases the repressor from the nucleoprotein complex and leaves the cAMP-CRP activator bound to its two DNA targets. Thus, cytidine interferes with the cooperative DNA binding of cAMP-CRP and CytR to tsx-p2. We characterized four tsx-p2 mutants exhibiting a reduced response to CytR; three carried mutations in the CRP-2 site, and one carried a mutation in the region between CRP-1 and the -10 sequence. Formation of the cAMP-CRP-CytR DNA nucleoprotein complex in vitro was perturbed in each mutant. These data indicate that the CytR repressor relies on the presence of the cAMP-CRP activator complex to regulate tsx-p2 promoter activity and that the formation of an active repression complex requires the combined interactions of cAMP-CRP and CytR at tsx-p2.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-16453417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-1647022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-1649947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-1962841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-212715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2162467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2170326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2265760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2575702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2644244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-265521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2673007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2673223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2674901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2826388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2844254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2989795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-2998936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-3073188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-3276691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-3515317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-3536129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6090927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6323249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6323997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6327627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6329724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6372090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1715855-6989335
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Receptor Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CytR protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Virus,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tsx protein, E coli
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5419-30
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:1715855-Amino Acid Sequence,
pubmed-meshheading:1715855-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1715855-Bacterial Proteins,
pubmed-meshheading:1715855-Base Sequence,
pubmed-meshheading:1715855-Carrier Proteins,
pubmed-meshheading:1715855-Cyclic AMP,
pubmed-meshheading:1715855-Cyclic AMP Receptor Protein,
pubmed-meshheading:1715855-DNA, Bacterial,
pubmed-meshheading:1715855-DNA Fingerprinting,
pubmed-meshheading:1715855-DNA-Binding Proteins,
pubmed-meshheading:1715855-Escherichia coli,
pubmed-meshheading:1715855-Escherichia coli Proteins,
pubmed-meshheading:1715855-Genes, Bacterial,
pubmed-meshheading:1715855-Molecular Sequence Data,
pubmed-meshheading:1715855-Mutation,
pubmed-meshheading:1715855-Plasmids,
pubmed-meshheading:1715855-Promoter Regions, Genetic,
pubmed-meshheading:1715855-RNA, Bacterial,
pubmed-meshheading:1715855-Receptors, Cyclic AMP,
pubmed-meshheading:1715855-Receptors, Virus,
pubmed-meshheading:1715855-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1715855-Repressor Proteins
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pubmed:year |
1991
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pubmed:articleTitle |
The cyclic AMP (cAMP)-cAMP receptor protein complex functions both as an activator and as a corepressor at the tsx-p2 promoter of Escherichia coli K-12.
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pubmed:affiliation |
Department of Biology, University of Konstanz, Germany.
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pubmed:publicationType |
Journal Article
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