Linked Life Data

1714432

Source:http://linkedlifedata.com/resource/pubmed/id/1714432

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1991-9-13
pubmed:abstractText
Peptide antisera made against the putative MP18 sequence recognizes an 18-kilodalton protein on western blots of bovine lens membrane. These peptide antisera were used to investigate possible age-related changes in the MP18 molecule in membrane fractions from newborn and adult bovine lenses. Western blot analysis of membrane fractions with peptide antisera to sequences 45-50 (anti-45), 113-129 (anti-113), and C-terminus 169-173 (anti-Ct) showed similar patterns. All three antisera reacted more strongly with MP18 from membrane prepared from newborn lenses than with corresponding preparations from the adult lens. Binding ratios of anti-45 to anti-Ct showed a statistically significant difference between cortex and nucleus of newborn lenses (P less than 0.02) and between cortex of newborn and cortex of adult lenses (P less than 0.001). Differences in binding ratios of anti-113 to anti-Ct were the most striking, with anti-113 unable to detect MP18 in membrane preparations from adult lenses. Collectively, these results confirmed the putative sequence of MP18 and demonstrated covalent changes in this molecule during the process of aging in the normal bovine lens.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2588-92
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Age-dependent covalent changes in MP18 from bovine lens membrane.
pubmed:affiliation
Division of Biology, Kansas State University, Manhattan 66506.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't