Linked Life Data

17118605

Source:http://linkedlifedata.com/resource/pubmed/id/17118605

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-2-26
pubmed:abstractText
Quantitative analysis by (1)H NMR is often hampered by heavily overlapping signals that may occur for complex mixtures, especially those containing similar compounds. Bidimensional homonuclear NMR spectroscopy can overcome this difficulty. A thorough review of acquisition and post-processing parameters was carried out to obtain accurate and precise, quantitative 2D J-resolved and DQF-COSY spectra in a much reduced time, thus limiting the spectrometer instabilities in the course of time. The number of t(1) increments was reduced as much as possible, and standard deviation was improved by optimization of spectral width, number of transients, phase cycling and apodization function. Localized polynomial baseline corrections were applied to the relevant chemical shift areas. Our method was applied to tropine-nortropine mixtures. Quantitative J-resolved spectra were obtained in less than 3 min and quantitative DQF-COSY spectra in 12 min, with an accuracy of 3% for J-spectroscopy and 2% for DQF-COSY, and a standard deviation smaller than 1%.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1243-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Optimization of homonuclear 2D NMR for fast quantitative analysis: application to tropine-nortropine mixtures.
pubmed:affiliation
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes, UMR CNRS 6006, Université de Nantes, Faculté des Sciences, F-44322 Nantes Cedex 03, France. patrick.giraudeau@univ-nantes.fr
pubmed:publicationType
Journal Article