Linked Life Data

17075797

Source:http://linkedlifedata.com/resource/pubmed/id/17075797

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-3-27
pubmed:abstractText
The orange-spotted grouper Epinephelus coioides is a protogynous hermaphroditic fish, but the physiological basis of its sex change remains largely unknown. In the present study, the 2-year-old orange-spotted grouper was induced to change sex precociously by oral administration of 17alpha-methyltestosterone (MT, 50 mg/Kg diet, twice a day at daily ration of 5% bodyweight) for 60 days. The serum testosterone levels were significantly elevated after MT treatment for 20 and 40 days as compared to control, but the levels of serum estradiol (E(2)) remained unchanged. The expression of P450aromA in the gonad significantly decreased after MT treatment for 20, 40, and 60 days. Accordingly, the enzyme activity of gonadal aromatase was also lower. The expression of FSHbeta subunit in the pituitary was significantly decreased after MT treatment for 20 days, but returned to the control levels after 40 and 60 days; however, the expression of LHbeta subunit was not altered significantly by MT treatment. The expression of FTZ-F1 in the gonad also decreased significantly in response to MT treatment for 40 and 60 days, but its expression in the pituitary was not altered significantly. Interestingly, when tested in vitro on ovarian fragments, MT had no direct effect on the expression of P450aromA and FTZ-F1 as well as the activity of gonadal aromatase, suggesting that the inhibition of gonadal P450aromatase and FTZ-F1 by MT may be mediated at upper levels of the brain-pituitary-gonadal axis. Taken together, these results indicated that FSH, P450aromA, FTZ-F1, and serum testosterone are associated with the MT-induced sex change of the orange-spotted grouper, but the cause-effect relationship between these factors and sex change in this species remains to be characterized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
(c) 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
665-73
pubmed:dateRevised
2010-12-1
pubmed:meshHeading
pubmed-meshheading:17075797-Animals, pubmed-meshheading:17075797-Aromatase, pubmed-meshheading:17075797-Bass, pubmed-meshheading:17075797-Estradiol, pubmed-meshheading:17075797-Female, pubmed-meshheading:17075797-Gene Expression, pubmed-meshheading:17075797-Gonadotropins, pubmed-meshheading:17075797-Gonads, pubmed-meshheading:17075797-Hermaphroditic Organisms, pubmed-meshheading:17075797-Homeodomain Proteins, pubmed-meshheading:17075797-Male, pubmed-meshheading:17075797-Methyltestosterone, pubmed-meshheading:17075797-Pituitary Gland, pubmed-meshheading:17075797-Protein Subunits, pubmed-meshheading:17075797-RNA, Messenger, pubmed-meshheading:17075797-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:17075797-Sex Determination Processes, pubmed-meshheading:17075797-Sex Differentiation, pubmed-meshheading:17075797-Steroidogenic Factor 1, pubmed-meshheading:17075797-Testosterone, pubmed-meshheading:17075797-Transcription Factors
pubmed:year
2007
pubmed:articleTitle
The mRNA expression of P450 aromatase, gonadotropin beta-subunits and FTZ-F1 in the orange-spotted grouper (Epinephelus Coioides) during 17alpha-methyltestosterone-induced precocious sex change.
pubmed:affiliation
Institute of Aquatic Economic Animals, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, Guangzhou, P.R.China ls68@zsu.edu.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't