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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1991-4-15
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pubmed:abstractText |
To evaluate the importance of tubular proteinuria in diabetic nephropathy, we studied the serial changes of micro-albuminuria, microproteinuria and protein patterns by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in 38 diabetic patients over 8 months. There was a significant correlation between the amount of micro-albuminuria measured by radio-immunoassay and the amount of microproteinuria quantitated by the Coomassie brilliant blue dye binding method (r = 0.976; p less than 0.0001). Micro-albuminuria of 50 mg/day was equivalent to microproteinuria of 190 mg/day. Among the 38 diabetic patients, 26 had micro-albuminuria above 50 mg/day, while 12 had micro-albuminuria below this level. There was a significant correlation between the amount of microproteinuria and haemoglobin A1, showing that the quantity of microproteinuria was affected by metabolic control. Diabetic patients with micro-albuminuria of above 50 mg/day have a significantly higher diastolic blood pressure than those below this level. Among the diabetic patients with micro-albuminuria of less than 50 mg/day, the amount of micro-albuminuria and microproteinuria remained constant, whereas progressive increases in micro-albuminuria and microproteinuria were observed among the 12 diabetic subjects with micro-albuminuria above 50 mg/day. These support the prognostic importance of this quantity of micro-albuminuria. The protein patterns as revealed by SDS-PAGE with Coomassie blue staining show a significant loss of low-molecular-weight proteins in 7 patients, which may therefore suggest tubular damage. The loss of tubular proteins persisted over a period of 8 months in all 7 subjects, and the amount gradually increased over this period.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0250-8095
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-81
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pubmed:dateRevised |
2007-2-14
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pubmed:meshHeading |
pubmed-meshheading:1706139-Adult,
pubmed-meshheading:1706139-Albuminuria,
pubmed-meshheading:1706139-Diabetic Nephropathies,
pubmed-meshheading:1706139-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1706139-Female,
pubmed-meshheading:1706139-Humans,
pubmed-meshheading:1706139-Longitudinal Studies,
pubmed-meshheading:1706139-Male,
pubmed-meshheading:1706139-Proteinuria,
pubmed-meshheading:1706139-Radioimmunoassay,
pubmed-meshheading:1706139-Rosaniline Dyes,
pubmed-meshheading:1706139-Staining and Labeling,
pubmed-meshheading:1706139-Time Factors
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pubmed:year |
1990
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pubmed:articleTitle |
Eight-month longitudinal study of urinary excretion of albumin and tubular proteins in diabetic subjects.
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pubmed:affiliation |
Department of Medicine, University of Hong Kong.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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