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rdf:type |
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|
lifeskim:mentions |
|
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pubmed:issue |
3
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pubmed:dateCreated |
1991-3-27
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pubmed:abstractText |
Transforming growth factor beta 1 (TGF-beta 1) is known to inhibit hepatocyte growth in vitro and in vivo. In this study, we analyzed the effect of TGF-beta 1 on alpha-fetoprotein (AFP) and albumin gene expression in HuH-7 human hepatoma cells. TGF-beta 1 inhibited cell growth in a dose dependent manner. The cellular secretion rate of AFP but not albumin was suppressed significantly by TGF-beta 1. TGF-beta 1 caused a significant reduction in the level of AFP mRNA. In contrast, the levels of albumin mRNA or beta-actin mRNA were not changed by TGF-beta 1. In transient transfection experiments, TGF-beta 1 resulted in selective repression of AFP promoter activity. These results suggest that TGF-beta 1 is one of the key factors involved in the differential regulation of the AFP gene and the albumin gene.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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|
pubmed:chemical |
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|
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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|
pubmed:issn |
0006-291X
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
14
|
|
pubmed:volume |
174
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|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
N
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pubmed:pagination |
1294-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1705121-Carcinoma, Hepatocellular,
pubmed-meshheading:1705121-Cell Division,
pubmed-meshheading:1705121-Cell Line,
pubmed-meshheading:1705121-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:1705121-Humans,
pubmed-meshheading:1705121-Liver Neoplasms,
pubmed-meshheading:1705121-RNA, Messenger,
pubmed-meshheading:1705121-Recombinant Fusion Proteins,
pubmed-meshheading:1705121-Serum Albumin,
pubmed-meshheading:1705121-Transfection,
pubmed-meshheading:1705121-Transforming Growth Factor beta,
pubmed-meshheading:1705121-alpha-Fetoproteins
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pubmed:year |
1991
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|
pubmed:articleTitle |
Transforming growth factor beta 1 differentially regulates alpha-fetoprotein and albumin in HuH-7 human hepatoma cells.
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pubmed:affiliation |
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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