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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12-13
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pubmed:dateCreated |
2006-11-10
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pubmed:abstractText |
Helper T lymphocytes that control CD8(+) T-cell and antibody responses are key elements for the resolution of infection by the hepatitis B virus and for the development of effective immunological memory after hepatitis B vaccination. We have used H-2 class II-deficient mice that express the human MHC class II molecule, HLA-DR1, to identify novel hepatitis B virus envelope-derived T helper epitopes. We confirmed the immunogenicity of a previously described HLA-DR1-restricted epitope, and identified three novel epitopes. CD4(+) T-cell immune responses against these epitopes were detected in peripheral blood mononuclear cells from HLA-DR1(+) individuals vaccinated against hepatitis B. We showed that subjects receiving the currently available hepatitis B vaccines do not develop cross-reactive T helper responses against one of the novel epitopes which are structurally variable between different hepatitis B virus subtypes. These findings highlight the need for developing vaccines against a wider range of viral subtypes, and establish humanized mice as a convenient tool for identifying new immunogenic epitopes from pathogens.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1286-4579
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2783-90
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pubmed:meshHeading |
pubmed-meshheading:17045504-Adolescent,
pubmed-meshheading:17045504-Adult,
pubmed-meshheading:17045504-Animals,
pubmed-meshheading:17045504-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17045504-Epitope Mapping,
pubmed-meshheading:17045504-Epitopes, T-Lymphocyte,
pubmed-meshheading:17045504-Female,
pubmed-meshheading:17045504-HLA-DR1 Antigen,
pubmed-meshheading:17045504-Hepatitis B,
pubmed-meshheading:17045504-Hepatitis B Surface Antigens,
pubmed-meshheading:17045504-Hepatitis B Vaccines,
pubmed-meshheading:17045504-Hepatitis B virus,
pubmed-meshheading:17045504-Humans,
pubmed-meshheading:17045504-Lymphocyte Activation,
pubmed-meshheading:17045504-Mice,
pubmed-meshheading:17045504-Mice, Transgenic,
pubmed-meshheading:17045504-Middle Aged,
pubmed-meshheading:17045504-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:17045504-Vaccines, DNA,
pubmed-meshheading:17045504-Viral Envelope Proteins
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pubmed:year |
2006
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pubmed:articleTitle |
Identification of novel HLA-DR1-restricted epitopes from the hepatitis B virus envelope protein in mice expressing HLA-DR1 and vaccinated human subjects.
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pubmed:affiliation |
Unité d'Immunité Cellulaire Antivirale, Département d'Immunologie, Institut Pasteur, 25-28 rue du Dr. Roux, 75015 Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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