Linked Life Data

16951144

Source:http://linkedlifedata.com/resource/pubmed/id/16951144

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2006-9-4
pubmed:abstractText
Despite the role of DNMT3B in de novo DNA methylation, a correlation between DNMT3B expression and promoter DNA methylation has not being established in tumors. We recently reported DeltaDNMT3B, a subfamily of DNMT3B, with seven variants, as the predominant expression forms in non-small cell lung cancer (NSCLC). We hypothesized that expression of the DeltaDNMT3B variants plays a role in promoter methylation formation during lung tumorigenesis. Expression of seven DeltaDNMT3B variants was measured in 119 NSCLCs and the corresponding normal lungs using reverse transcription-PCR. The expression patterns of DeltaDNMT3B variants were analyzed with the status of p16 and RASSF1A promoter methylation in the tumors as well as in patients' clinical variables, including outcomes. Expression of DeltaDNMT3B variants was detected in 94 of 119 (80%) tumors but in only 22 (18%) of the corresponding normal lungs (P < 0.0001). DeltaDNMT3B1, DeltaDNMT3B2, and DeltaDNMT3B4 were the most frequently detected transcripts in the tumors (62%, 76%, and 46%, respectively). The expression of DeltaDNMT3B variants was associated with p16 and RASSF1A promoter methylation in the tumors, but the strongest association was between DeltaDNMT3B4 and RASSF1A. Forty-two of 46 (91%) tumors with RASSF1A promoter methylation expressed DeltaDNMT3B4 compared with only 13 of 73 (18%) tumors without the promoter methylation (P < 0.0001). Strong associations were also observed between expression of the variants in the tumors and in patients' clinical outcomes. Expression of DeltaDNMT3B variants is common in NSCLC and may play an important role in the development of promoter methylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1538-7445
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8361-6
pubmed:dateRevised
2010-5-21
pubmed:meshHeading
pubmed-meshheading:16951144-Adult, pubmed-meshheading:16951144-Aged, pubmed-meshheading:16951144-Aged, 80 and over, pubmed-meshheading:16951144-Carcinoma, Non-Small-Cell Lung, pubmed-meshheading:16951144-DNA, Neoplasm, pubmed-meshheading:16951144-DNA (Cytosine-5-)-Methyltransferase, pubmed-meshheading:16951144-DNA Methylation, pubmed-meshheading:16951144-Genes, p16, pubmed-meshheading:16951144-Genetic Variation, pubmed-meshheading:16951144-Humans, pubmed-meshheading:16951144-Lung Neoplasms, pubmed-meshheading:16951144-Middle Aged, pubmed-meshheading:16951144-Neoplasm Staging, pubmed-meshheading:16951144-Promoter Regions, Genetic, pubmed-meshheading:16951144-RNA, Neoplasm, pubmed-meshheading:16951144-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16951144-Tumor Suppressor Proteins
pubmed:year
2006
pubmed:articleTitle
Expression of Delta DNMT3B variants and its association with promoter methylation of p16 and RASSF1A in primary non-small cell lung cancer.
pubmed:affiliation
Molecular Biology Laboratory, Department of Thoracic/Head and Neck Medical Oncology, Department of Thoracic, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.