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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-9-1
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pubmed:abstractText |
In this study, the effects of 4-tert-octylphenol (OP) were examined on the viability of rat cultured Sertoli cells using the MTT assay and OP-induced apoptosis was detected by transmission electron microscope (TEM), flow cytometric analysis and Hoechst staining. In addition, RT-PCR was used to analyze the levels of Bcl-2 and Bax mRNA. Bcl-2, Bax and caspase-3 protein expressions were determined by Western blot analysis. Sertoli cells were treated with OP from 30 to 60microM for 6-24h. Decreased viability of Sertoli cells and increased apoptosis occurred in a concentration- and a time-dependent manner. The expression of Bcl-2 was down-regulated, while the expression of Bax up-regulated. OP also down-regulated the expression of 32kDa procaspase-3, which was cleaved to generate active subunit (17kDa). These results suggest that OP may induce Sertoli cell apoptosis by regulation of Bcl-2/Bax and caspase-3 activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-tert-octylphenol,
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Environmental Pollutants,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0378-4274
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
166
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
178-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16893618-Animals,
pubmed-meshheading:16893618-Apoptosis,
pubmed-meshheading:16893618-Blotting, Western,
pubmed-meshheading:16893618-Caspase 3,
pubmed-meshheading:16893618-Caspases,
pubmed-meshheading:16893618-Cell Survival,
pubmed-meshheading:16893618-Cells, Cultured,
pubmed-meshheading:16893618-Dose-Response Relationship, Drug,
pubmed-meshheading:16893618-Environmental Pollutants,
pubmed-meshheading:16893618-Flow Cytometry,
pubmed-meshheading:16893618-Male,
pubmed-meshheading:16893618-Microscopy, Electron, Transmission,
pubmed-meshheading:16893618-Phenols,
pubmed-meshheading:16893618-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:16893618-Rats,
pubmed-meshheading:16893618-Rats, Sprague-Dawley,
pubmed-meshheading:16893618-Sertoli Cells,
pubmed-meshheading:16893618-Time Factors,
pubmed-meshheading:16893618-bcl-2-Associated X Protein
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pubmed:year |
2006
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pubmed:articleTitle |
Octylphenol induces apoptosis in cultured rat Sertoli cells.
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pubmed:affiliation |
Key Laboratory of Reproductive Medicine of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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