Linked Life Data

16817902

Source:http://linkedlifedata.com/resource/pubmed/id/16817902

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2006-7-4
pubmed:abstractText
N-6 polyunsaturated fatty acids (PUFAs) may be associated with increased risk of colon cancer, whereas n-3 PUFAs may have a protective effect. We examined the effects of docosahexaenoic acid (DHA), eicosapentaenoic acid and arachidonic acid on the colon carcinoma cell lines SW480 derived from a primary tumour, and SW620 derived from a metastasis of the same tumour. DHA had the strongest growth-inhibitory effect on both cell lines. SW620 was relatively more growth-inhibited than SW480, but SW620 also had the highest growth rate in the absence of PUFAs. Flow cytometry revealed an increase in the fraction of cells in the G2/M phase of the cell cycle, particularly for SW620 cells. Growth inhibition was apparently not caused by increased lipid peroxidation, reduced glutathione or low activity of glutathione peroxidase. Transmission electron microscopy revealed formation of cytoplasmic lipid droplets after DHA treatment. In SW620 cells an eightfold increase in total cholesteryl esters and a 190-fold increase in DHA-containing cholesteryl esters were observed after DHA treatment. In contrast, SW480 cells accumulated DHA-enriched triglycerides. Arachidonic acid accumulated in a similar manner, whereas the nontoxic oleic acid was mainly incorporated in triglycerides in both cell lines. Interestingly, nuclear sterol regulatory element-binding protein 1 (nSREBP1), recently associated with cell growth regulation, was downregulated after DHA treatment in both cell lines. Our results demonstrate cell-specific mechanisms for the processing and storage of cytotoxic PUFAs in closely related cell lines, and suggest downregulation of nSREBP1 as a possible contributor to the growth inhibitory effect of DHA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1742-464X
pubmed:author
pubmed:issnType
Print
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2749-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16817902-Apoptosis, pubmed-meshheading:16817902-Cell Cycle, pubmed-meshheading:16817902-Cell Line, Tumor, pubmed-meshheading:16817902-Colonic Neoplasms, pubmed-meshheading:16817902-Docosahexaenoic Acids, pubmed-meshheading:16817902-Down-Regulation, pubmed-meshheading:16817902-Eicosanoids, pubmed-meshheading:16817902-Enzyme Inhibitors, pubmed-meshheading:16817902-Fatty Acids, Unsaturated, pubmed-meshheading:16817902-Glutathione, pubmed-meshheading:16817902-Humans, pubmed-meshheading:16817902-Lipid Metabolism, pubmed-meshheading:16817902-Lipid Peroxidation, pubmed-meshheading:16817902-Oxidants, pubmed-meshheading:16817902-RNA, Messenger, pubmed-meshheading:16817902-Sterol O-Acyltransferase, pubmed-meshheading:16817902-Sterol Regulatory Element Binding Protein 1, pubmed-meshheading:16817902-Time Factors, pubmed-meshheading:16817902-Tumor Cells, Cultured, pubmed-meshheading:16817902-Vitamin E
pubmed:year
2006
pubmed:articleTitle
Closely related colon cancer cell lines display different sensitivity to polyunsaturated fatty acids, accumulate different lipid classes and downregulate sterol regulatory element-binding protein 1.
pubmed:affiliation
Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway. svanhild.schonberg@ntnu.no
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't