rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2006-6-23
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pubmed:abstractText |
The present study has demonstrated a differential cytotoxicity of stellettin A (1) between human leukemia HL-60 cells (IC50 0.4 microg/mL) and human prostate cancer LNCaP cells (IC50 120 microg/mL). Treatment of cells with 1 revealed the activation of NADPH oxidase, the dramatic generation of reactive oxygen species, and the dissipation of mitochondrial membrane potentials, with HL-60 cells being more sensitive than LNCaP cells by an order of magnitude. Immunoblotting analysis further demonstrated a stronger upregulation of the apoptosis marker proteins, FasL and caspase-3, in HL-60 cells, and pretreatment of cells with antisense oligonucleotide for caspase-3 abolished apoptosis. All available evidence suggests that 1 induces oxidative cell death through a FasL-caspase-3-apoptotic pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Triterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factors,
http://linkedlifedata.com/resource/pubmed/chemical/stellettin A
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0163-3864
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
934-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16792413-Animals,
pubmed-meshheading:16792413-Apoptosis,
pubmed-meshheading:16792413-Caspase 3,
pubmed-meshheading:16792413-Caspases,
pubmed-meshheading:16792413-Fas Ligand Protein,
pubmed-meshheading:16792413-HL-60 Cells,
pubmed-meshheading:16792413-Humans,
pubmed-meshheading:16792413-Male,
pubmed-meshheading:16792413-Membrane Glycoproteins,
pubmed-meshheading:16792413-Membrane Potentials,
pubmed-meshheading:16792413-Mitochondria,
pubmed-meshheading:16792413-Oxidative Stress,
pubmed-meshheading:16792413-Porifera,
pubmed-meshheading:16792413-Prostatic Neoplasms,
pubmed-meshheading:16792413-Triterpenes,
pubmed-meshheading:16792413-Tumor Cells, Cultured,
pubmed-meshheading:16792413-Tumor Necrosis Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Stellettin A induces oxidative stress and apoptosis in HL-60 human leukemia and LNCaP prostate cancer cell lines.
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pubmed:affiliation |
Department of Anatomy and School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, People's Republic of China. ken-liu@cuhk.edu.hk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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