Linked Life Data

16772430

Source:http://linkedlifedata.com/resource/pubmed/id/16772430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2006-7-14
pubmed:abstractText
Placental trophoblast cells are unique endocrine cells that play vital roles during the processes of embryonic implantation and placentation. However, research into the function of human trophoblast has been largely restrained mainly due to a lack of adequate cell models. A normal placenta-origin cytotrophoblast cell line (NPC) was previously established by our group, but these cells showed replicating senescence after 50 population doublings (PDs). In this study, the human telomerase catalytic subunit gene (htert) was transferred into B6 strain of NPC cells, and strains with reconstituted telomerase activity (B6Tert) were established. It was shown that B6Tert-1 cells produce various biomarkers of normal extravillous cytotrophoblasts during the early weeks of gestation. Meanwhile, the cell invasiveness was inhibited by transforming growth factor beta (TGFbeta). However, their ability to form syncytium was relatively low when stimulated with fetal calf serum (FCS). The cells maintained normal cell growth properties and failed to elicit tumours in nude mice. They proliferated continuously with no signs of senescence until the final count at 210 PDs. The growth rate of B6Tert-1 cells was increased when compared with the parental cells, which results, at least partly, from facilitating release of the G1/S checkpoint during the cell-cycle regulation. This is the first report of immortalizing human normal cytotrophoblast (CTB) cells by activation of telomerase activity. The cells will provide an ideal in vitro model for the study of human extravillous trophoblast (EVT) functions and consequently the mechanisms of embryonic implantation and placentation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1360-9947
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16772430-Animals, pubmed-meshheading:16772430-Blotting, Northern, pubmed-meshheading:16772430-Blotting, Western, pubmed-meshheading:16772430-Cell Line, pubmed-meshheading:16772430-DNA-Binding Proteins, pubmed-meshheading:16772430-Female, pubmed-meshheading:16772430-Humans, pubmed-meshheading:16772430-Immunohistochemistry, pubmed-meshheading:16772430-Karyotyping, pubmed-meshheading:16772430-Keratins, pubmed-meshheading:16772430-Mice, pubmed-meshheading:16772430-Mice, Inbred BALB C, pubmed-meshheading:16772430-Mice, Nude, pubmed-meshheading:16772430-Microscopy, Phase-Contrast, pubmed-meshheading:16772430-Neoplasms, Experimental, pubmed-meshheading:16772430-Pregnancy, pubmed-meshheading:16772430-RNA, Messenger, pubmed-meshheading:16772430-Retinoblastoma Protein, pubmed-meshheading:16772430-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16772430-Telomerase, pubmed-meshheading:16772430-Telomere, pubmed-meshheading:16772430-Transfection, pubmed-meshheading:16772430-Trophoblasts, pubmed-meshheading:16772430-Tumor Suppressor Protein p53, pubmed-meshheading:16772430-Vimentin
pubmed:year
2006
pubmed:articleTitle
Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity.
pubmed:affiliation
State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. wangyl@ioz.ac.cn
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't