Linked Life Data

16740655

Source:http://linkedlifedata.com/resource/pubmed/id/16740655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2006-9-27
pubmed:abstractText
MUC1 expression responds differently to changes in progesterone (P) levels in mouse vs. human uterine epithelium. Two isoforms of progesterone receptor, PRA and PRB, mediate the physiological effects of P. Using transient transfection of a human uterine epithelial cell line, HEC-1A, we showed that liganded PRB stimulated MUC1 gene activity. PRA alone had little effect on MUC1 promoter activity, but antagonized the PRB-mediated stimulation. The region from 523 to 570 bp upstream of the transcriptional start site was shown to be required for the P response. Mutation of two potential P-responsive element (PRE) half-sites in this region partially inhibited the PRB-mediated response, and one PRE half-site disrupted binding of both PRB and PRA to a consensus PRE in an EMSA. These along with other studies indicated that multiple cis elements in the -523- to -570-bp region cooperate to mediate P responsiveness, and that PR interaction with other transcription factors in this region is likely. Using ovariectomized wild-type, PR knockout (PRKO), PRAKO, and PRBKO mice, P antagonism of estrogen-stimulated Muc1 protein and mRNA expression was shown to be dependent on PRA. In summary, these data show that liganded PRB stimulates MUC1 expression in human uterine epithelial cells, whereas liganded PRA antagonizes MUC1 expression in both human and mouse uterine epithelial cells. The differential MUC1 response to P in these two species may be due to dissimilar expression of the two PR isoforms in the uterine epithelium.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2278-91
pubmed:dateRevised
2011-11-7
pubmed:meshHeading
pubmed-meshheading:16740655-Animals, pubmed-meshheading:16740655-Antigens, Neoplasm, pubmed-meshheading:16740655-Blotting, Western, pubmed-meshheading:16740655-Cell Line, Tumor, pubmed-meshheading:16740655-Chromatin Immunoprecipitation, pubmed-meshheading:16740655-DNA Primers, pubmed-meshheading:16740655-Electrophoretic Mobility Shift Assay, pubmed-meshheading:16740655-Epithelial Cells, pubmed-meshheading:16740655-Female, pubmed-meshheading:16740655-Gene Expression Regulation, pubmed-meshheading:16740655-Humans, pubmed-meshheading:16740655-Immunohistochemistry, pubmed-meshheading:16740655-Mice, pubmed-meshheading:16740655-Mice, Knockout, pubmed-meshheading:16740655-Mucin-1, pubmed-meshheading:16740655-Mucins, pubmed-meshheading:16740655-Mutagenesis, pubmed-meshheading:16740655-Plasmids, pubmed-meshheading:16740655-Promoter Regions, Genetic, pubmed-meshheading:16740655-Receptors, Progesterone, pubmed-meshheading:16740655-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16740655-Transfection, pubmed-meshheading:16740655-Uterus
pubmed:year
2006
pubmed:articleTitle
Progesterone receptor isoforms A and B differentially regulate MUC1 expression in uterine epithelial cells.
pubmed:affiliation
Department of Biological Sciences, University of Delaware, 118C Wolf Hall, Newark, Delaware 19713, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural