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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 5
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pubmed:dateCreated |
2006-5-9
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pubmed:abstractText |
The large subunit of the essential pre-mRNA splicing factor U2 auxiliary factor (U2AF65) binds the polypyrimidine tract near the 3' splice site of pre-mRNA introns and directs the association of the U2 small nuclear ribonucleoprotein particle (U2 snRNP) of the spliceosome with the pre-mRNA. Protein engineering, in which the flexible linker region connecting tandem RNA-recognition motifs (RRMs) within the U2AF65 RNA-binding domain was partially deleted, allowed successful crystallization of the protein-nucleic acid complex. Cocrystals of a U2AF65 variant with a deoxyuridine dodecamer diffract X-rays to 2.9 angstroms resolution and contain one complex per asymmetric unit.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-10217141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-10449418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-10499800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-10864047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-11175909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-12454499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-14730023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-1538748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-15769473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-15853797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-16179478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-2314278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-2531895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-2963698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-5700707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-7535921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-7680770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-7761834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-7984237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-8474334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682775-9671485
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
457-9
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pubmed:dateRevised |
2011-5-5
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Crystallization and preliminary X-ray analysis of a U2AF65 variant in complex with a polypyrimidine-tract analogue by use of protein engineering.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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