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pubmed-article:16660311pubmed:dateCreated2010-6-29lld:pubmed
pubmed-article:16660311pubmed:abstractTextA uracil phosphoribosyltransferase (UMP-pyrophosphorylase) was found in several angiosperms and was partially purified from epicotyls of pea (Pisum sativum L. cv. Alaska) seedlings. Its pH optimum was about 8.5; its required approximately 0.3 mm MgCl(2) for maximum activity but was inhibited by MnCl(2); its molecular weight determined by chromatography on Sephadex G-150 columns was approximately 100,000; its K(m) values for uracil and 5-phosphorylribose 1-pyrophosphate were 0.7 mum and 11 mum; and it was partially resolved from a similar phosphoribosyltransferase converting orotic acid to orotodine 5'-phosphate. Enzyme fractions containing both uracil phosphoribosyl transferase and orotate phosphoribosyltransferase converted 6-azauracil and 5-fluorouracil to products with chromatographic properties of 6-azauradine 5'-phosphate and 5-fluorouridine 5'-phosphate. Uracil phosphoribosyltransferase probably functions in salvage of uracil for synthesis of pyrimidine nucleotides.lld:pubmed
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pubmed-article:16660311pubmed:statusPubMed-not-MEDLINElld:pubmed
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pubmed-article:16660311pubmed:issn0032-0889lld:pubmed
pubmed-article:16660311pubmed:authorpubmed-author:BressanR ARAlld:pubmed
pubmed-article:16660311pubmed:authorpubmed-author:RossC WCWlld:pubmed
pubmed-article:16660311pubmed:authorpubmed-author:MurrayM GMGlld:pubmed
pubmed-article:16660311pubmed:authorpubmed-author:GaleJ MJMlld:pubmed
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pubmed-article:16660311pubmed:volume61lld:pubmed
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pubmed-article:16660311pubmed:pagination442-6lld:pubmed
pubmed-article:16660311pubmed:dateRevised2010-9-14lld:pubmed
pubmed-article:16660311pubmed:year1978lld:pubmed
pubmed-article:16660311pubmed:articleTitleProperties of pea seedling uracil phosphoribosyltransferase and its distribution in other plants.lld:pubmed
pubmed-article:16660311pubmed:affiliationDepartment of Botany and Plant Pathology, Colorado State University, Fort Collins, Colorado 80523.lld:pubmed
pubmed-article:16660311pubmed:publicationTypeJournal Articlelld:pubmed
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