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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0033684,
umls-concept:C0080347,
umls-concept:C0171961,
umls-concept:C0205263,
umls-concept:C0205314,
umls-concept:C0271510,
umls-concept:C0679622,
umls-concept:C1333892,
umls-concept:C1335073,
umls-concept:C1367453,
umls-concept:C1417838,
umls-concept:C1551336,
umls-concept:C1823978,
umls-concept:C2698421
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pubmed:issue |
23
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pubmed:dateCreated |
2006-6-5
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pubmed:abstractText |
Kruppel-associated box (KRAB) domain-containing proteins consist of potential transcriptional repression modules. Previously, gonadotropin-inducible ovarian transcription factor-1 (GIOT-1) was identified as a novel KRAB-containing zinc finger protein and shown to have transcriptional repression activity. Here, we demonstrate that orphan nuclear receptor Nur77 regulates GIOT-1 gene expression in testicular Leydig cell lines and that GIOT-1 acts as a novel corepressor of the orphan nuclear receptor steroidogenic factor 1 (SF-1). Mutation analysis of the GIOT-1 promoter and overexpression analysis of dominant-negative Nur77 revealed that luteinizing hormone activates GIOT-1 gene expression through Nur77. Electrophoretic mobility shift and chromatin immunoprecipitation assays showed that Nur77 directly binds to the GIOT-1 promoter. GIOT-1 represses the SF-1 transactivation, and specific interaction between GIOT-1 and SF-1 was observed. We also demonstrate an interaction between GIOT-1 and histone deacetylase 2 (HDAC2). GIOT-1-mediated transrepression was recovered by down-regulation of HDAC2 expression with small interfering RNA of HDAC2. Knock down of the endogenous GIOT-1 results in significant enhancement of CYP17 expression in Leydig cells. In conclusion, this study of cross-talk between GIOT-1 and orphan nuclear receptors will provide new insights into the role of KRAB-containing zinc finger proteins in nuclear receptor action.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GIOT-1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hdac2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylases,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NR4A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NR5A1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nr4a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 4...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Steroid,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Steroidogenic Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/steroidogenic factor 1, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:AhnSeung-WonSW,
pubmed-author:ChoiHueng-SikHS,
pubmed-author:HongCheol YiCY,
pubmed-author:KeeHae JinHJ,
pubmed-author:KimHye-JinHJ,
pubmed-author:LeeIn-KyuIK,
pubmed-author:LeeKeesookK,
pubmed-author:LeeYong-SooYS,
pubmed-author:ParkYun-YongYY,
pubmed-author:SongKwang-HoonKH,
pubmed-author:TseLL
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15605-14
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16595694-Animals,
pubmed-meshheading:16595694-Base Sequence,
pubmed-meshheading:16595694-Chromatin Immunoprecipitation,
pubmed-meshheading:16595694-DNA,
pubmed-meshheading:16595694-DNA-Binding Proteins,
pubmed-meshheading:16595694-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16595694-Gene Expression Regulation,
pubmed-meshheading:16595694-Histone Deacetylase 2,
pubmed-meshheading:16595694-Histone Deacetylases,
pubmed-meshheading:16595694-Homeodomain Proteins,
pubmed-meshheading:16595694-Humans,
pubmed-meshheading:16595694-Male,
pubmed-meshheading:16595694-Mice,
pubmed-meshheading:16595694-Molecular Sequence Data,
pubmed-meshheading:16595694-Nuclear Receptor Subfamily 4, Group A, Member 1,
pubmed-meshheading:16595694-Promoter Regions, Genetic,
pubmed-meshheading:16595694-RNA, Small Interfering,
pubmed-meshheading:16595694-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:16595694-Receptors, Steroid,
pubmed-meshheading:16595694-Repressor Proteins,
pubmed-meshheading:16595694-Steroidogenic Factor 1,
pubmed-meshheading:16595694-Testis,
pubmed-meshheading:16595694-Transcription Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Orphan nuclear receptor Nur77 induces zinc finger protein GIOT-1 gene expression, and GIOT-1 acts as a novel corepressor of orphan nuclear receptor SF-1 via recruitment of HDAC2.
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pubmed:affiliation |
Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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