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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-3-31
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pubmed:abstractText |
Exposure of human non-small cell lung cancer cells (NCI-H460) to gradually increasing concentrations of doxorubicin resulted in the appearance of a new cell line (NCI-H460/R) that was resistant to doxorubicin (96.2-fold) and cross-resistant to etoposide, paclitaxel, vinblastine and epirubicin. Slight cross-resistance to two MDR-unrelated drugs 8-Cl-cAMP and sulfinosine was observed. Flow cytometry analysis showed that the accumulation of doxorubicin in the resistant cells was 88.4% lower than in the parental cells. Also, verapamil significantly decreased the efflux rate in NCI-H460 and NCI-H460/R cells, whereas curcumin inhibited the efflux in NCI-H460 cells only. Gene expression data confirmed the induction of mdr1 (P-gp), as judged by the observed 15-fold increase in its mRNA concentration in doxorubicin-resistant NCI-H460/R cells. In contrast, mrp1 and lrp expression was unaffected by the doxorubicin resistance. Further work should develop a rationale for a novel treatment of NSCLC with appropriate modulators of resistance aimed at improving the outcome of the acquired drug resistance.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Curcumin,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Etoposide,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Multidrug Resistance-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Paclitaxel,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamines,
http://linkedlifedata.com/resource/pubmed/chemical/Verapamil,
http://linkedlifedata.com/resource/pubmed/chemical/Vinblastine,
http://linkedlifedata.com/resource/pubmed/chemical/lissamine rhodamine B
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1120-009X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
66-73
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pubmed:dateRevised |
2009-8-4
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pubmed:meshHeading |
pubmed-meshheading:16572896-Antineoplastic Agents,
pubmed-meshheading:16572896-Carcinoma, Non-Small-Cell Lung,
pubmed-meshheading:16572896-Curcumin,
pubmed-meshheading:16572896-Doxorubicin,
pubmed-meshheading:16572896-Drug Resistance, Multiple,
pubmed-meshheading:16572896-Drug Resistance, Neoplasm,
pubmed-meshheading:16572896-Etoposide,
pubmed-meshheading:16572896-Glutathione Transferase,
pubmed-meshheading:16572896-Humans,
pubmed-meshheading:16572896-Lung Neoplasms,
pubmed-meshheading:16572896-Multidrug Resistance-Associated Proteins,
pubmed-meshheading:16572896-Paclitaxel,
pubmed-meshheading:16572896-RNA, Messenger,
pubmed-meshheading:16572896-RNA, Neoplasm,
pubmed-meshheading:16572896-Rhodamines,
pubmed-meshheading:16572896-Tumor Cells, Cultured,
pubmed-meshheading:16572896-Verapamil,
pubmed-meshheading:16572896-Vinblastine
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pubmed:year |
2006
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pubmed:articleTitle |
Induced resistance in the human non small cell lung carcinoma (NCI-H460) cell line in vitro by anticancer drugs.
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pubmed:affiliation |
Institute for Biological Research, Department of Neurobiology and Immunology, Laboratory of Molecular Neurobiology, Belgrade, Serbia & Montenegro.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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