Linked Life Data

1649505

Source:http://linkedlifedata.com/resource/pubmed/id/1649505

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-8-19
pubmed:databankReference
pubmed:abstractText
The hemagglutinin-esterase (HE) membrane glycoprotein is present only in some members of the coronavirus family, including some strains of mouse hepatitis virus (MHV). In the JHM strain of MHV, expression of the HE gene is variable and corresponds to the number of copies of a UCUAA pentanucleotide sequence present at the 3'-end of the leader RNA. This copy number varies among MHV strains, depending on their passage history. The JHM isolates with two copies of UCUAA in their leader RNA showed a high level of HE expression, whereas the JHM isolate with three copies had a low-level expression. In this study, the analysis of HE gene expression was extended to other MHV strains. The synthesis of HE mRNA in these viruses also correlates with the copy number of UCUAA in the leader RNA and the particular intergenic sequence preceding the HE gene. In one MHV strain, MHV-1, no detectable HE mRNA was synthesized, despite the presence of a proper transcription initiation signal. This lack of HE mRNA expression was consistent with a leader RNA containing three UCUAA copies. However, mutations and deletions within the coding region of the MHV-1 HE gene have generated a stretch of sequence which resembled the transcriptional initiation motif, and was shown to initiate the synthesis of a novel smaller mRNA. These findings strengthened the theory that interactions between leader RNA and transcriptional initiation sequences regulate MHV subgenomic mRNA transcription. Sequence analysis revealed that most MHV strains, through extensive mutations, deletions, or insertions, have lost the complete HE open reading frame, thus turning HE into a pseudogene. This high degree of variation is unusual as the other three structural proteins (spike, membrane, and nucleocapsid) are well-maintained. In contrast to bovine coronavirus, which apparently requires HE for viral replication, the HE protein in MHV may be only an accessory protein which is not necessary for viral replication. JHM and MHV-S, however, have preserved the expression of HE protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
647-57
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1649505-Amino Acid Sequence, pubmed-meshheading:1649505-Animals, pubmed-meshheading:1649505-Base Sequence, pubmed-meshheading:1649505-Cell Line, pubmed-meshheading:1649505-DNA, Viral, pubmed-meshheading:1649505-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:1649505-Gene Expression, pubmed-meshheading:1649505-Glycoproteins, pubmed-meshheading:1649505-Hemagglutinins, Viral, pubmed-meshheading:1649505-Introns, pubmed-meshheading:1649505-Mice, pubmed-meshheading:1649505-Molecular Sequence Data, pubmed-meshheading:1649505-Murine hepatitis virus, pubmed-meshheading:1649505-Mutation, pubmed-meshheading:1649505-RNA, Messenger, pubmed-meshheading:1649505-RNA, Viral, pubmed-meshheading:1649505-Sequence Homology, Nucleic Acid, pubmed-meshheading:1649505-Transcription, Genetic, pubmed-meshheading:1649505-Viral Fusion Proteins, pubmed-meshheading:1649505-Viral Proteins
pubmed:year
1991
pubmed:articleTitle
Heterogeneity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses.
pubmed:affiliation
Howard Hughes Medical Institute, University of Southern California, School of Medicine, Los Angeles 90033.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't