|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1991-8-14
|
|
pubmed:abstractText |
cDNAs encoding rat adipose tissue hormone-sensitive lipase were expressed in COS cells, under the control of the SV40 promoter to half the level in rat adipocytes, the richest native source of the enzyme. A cDNA lacking most of the long 5'-untranslated region of the full-length rat hormone-sensitive lipase cDNA was, with regard to the lipase activity, on the average 70% more efficiently expressed that the full-length cDNA. The recombinant protein was almost identical to hormone-sensitive lipase of rat adipose tissue with respect to specific activity, susceptibility to inhibitors, molecular size, phosphorylation and activation by cyclic AMP-dependent protein kinase. The described eukaryotic expression system will allow analysis of effects of amino acid substitutions introduced into the lipase molecule by site-directed mutagenesis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
8
|
|
pubmed:volume |
285
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
139-44
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:1648510-Adenosine Monophosphate,
pubmed-meshheading:1648510-Adipose Tissue,
pubmed-meshheading:1648510-Animals,
pubmed-meshheading:1648510-Blotting, Northern,
pubmed-meshheading:1648510-Cell Line, Transformed,
pubmed-meshheading:1648510-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:1648510-Isoflurophate,
pubmed-meshheading:1648510-Mercury,
pubmed-meshheading:1648510-Phosphorylation,
pubmed-meshheading:1648510-Precipitin Tests,
pubmed-meshheading:1648510-Protein Kinases,
pubmed-meshheading:1648510-Rats,
pubmed-meshheading:1648510-Recombinant Proteins,
pubmed-meshheading:1648510-Simian virus 40,
pubmed-meshheading:1648510-Sterol Esterase,
pubmed-meshheading:1648510-Transfection
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Expression of biologically active hormone-sensitive lipase in mammalian (COS) cells.
|
|
pubmed:affiliation |
Department of Medical and Physiological Chemistry 4, University of Lund, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
