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pubmed-article:16472650pubmed:abstractTextRac binds tightly to lipid membranes through a lipid modification. The influence of the lipid membrane environment on the multiple interactions of Rac has not been well documented. In this chapter, we detail a method to prepare geranyl-geranylated Rac bound to liposomes of defined composition. With this method, one can dissect some lipid-protein interactions that facilitate the interaction of Rac with other proteins such as guanine nucleotide exchange factors and bacterial toxins.lld:pubmed
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pubmed-article:16472650pubmed:authorpubmed-author:AntonnyBrunoBlld:pubmed
pubmed-article:16472650pubmed:authorpubmed-author:MesminBrunoBlld:pubmed
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pubmed-article:16472650pubmed:volume406lld:pubmed
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pubmed-article:16472650pubmed:pagination70-80lld:pubmed
pubmed-article:16472650pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16472650pubmed:year2006lld:pubmed
pubmed-article:16472650pubmed:articleTitleGEF and glucosylation assays on liposome-bound Rac.lld:pubmed
pubmed-article:16472650pubmed:affiliationCNRS-Institut de Pharmacologie, Moleculaire et Cellulaire, Valbonne, France.lld:pubmed
pubmed-article:16472650pubmed:publicationTypeJournal Articlelld:pubmed