Source:http://linkedlifedata.com/resource/pubmed/id/16440180
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2006-1-27
|
pubmed:abstractText |
Fluid and electrolyte absorption by colonic crypts depends on the transport properties of crypt cellular and paracellular routes and of the pericryptal sheath. As a low-Na(+) diet increases aldosterone and angiotensin II secretion, either hormone could affect absorption. Control and adrenalectomized (ADX) Sprague-Dawley rats were kept at a high-NaCl (HS) diet and then switched to low-NaCl (LS) diet for 3 days. Aldosterone or angiotensin II plasma concentrations were maintained using implanted osmotic mini-pumps. The extracellular Na(+) concentration in isolated rat distal colonic mucosa was determined by confocal microscopy using a low-affinity Na(+) -sensitive fluorescent dye (Sodium red, and Na(+) -insensitive BODIPY) bound to polystyrene beads. Crypt permeability to FITC-labelled dextran (10 kDa) was monitored by its rate of escape from the crypt lumen into the pericryptal space. Mucosal ion permeability was estimated by transepithelial electrical resistance (TER) and amiloride-sensitive short-circuit current (SCC). The epithelial Na(+) channel, ENaC, was determined by immunolocalization. LS diet decreased crypt wall permeability to dextran by 10-fold and doubled TER. Following ADX, aldosterone decreased crypt wall dextran permeability, increased TER, increased Na(+) accumulation in the pericryptal sheath and ENaC expression even in HS. Infusion of angiotensin II to ADX rats did not reverse the effects of aldosterone deprivation. These findings indicate that aldosterone alone is responsible for both the increase in Na(+) absorption and the decreased paracellular and pericryptal sheath permeability.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0022-2631
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
206
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
43-51
|
pubmed:dateRevised |
2007-8-13
|
pubmed:meshHeading |
pubmed-meshheading:16440180-Adaptation, Physiological,
pubmed-meshheading:16440180-Aldosterone,
pubmed-meshheading:16440180-Angiotensin II,
pubmed-meshheading:16440180-Animals,
pubmed-meshheading:16440180-Colon,
pubmed-meshheading:16440180-Diet, Sodium-Restricted,
pubmed-meshheading:16440180-Intestinal Absorption,
pubmed-meshheading:16440180-Male,
pubmed-meshheading:16440180-Permeability,
pubmed-meshheading:16440180-Rats,
pubmed-meshheading:16440180-Rats, Sprague-Dawley,
pubmed-meshheading:16440180-Sodium, Dietary
|
pubmed:year |
2005
|
pubmed:articleTitle |
Aldosterone reduces crypt colon permeability during low-sodium adaptation.
|
pubmed:affiliation |
Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain. mmoreto@ub.edu
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|