| pubmed-article:16282198 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C0032200 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C1709059 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:16282198 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:16282198 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16282198 | pubmed:dateCreated | 2006-3-13 | lld:pubmed |
| pubmed-article:16282198 | pubmed:abstractText | We showed previously that the expression of alpha(7)-integrin in aortic vascular smooth muscle cells (VSMC) is enhanced in a rat model of atherosclerosis. In the present study, we investigated the effects of platelet-derived growth factor (PDGF) on alpha(7)-integrin expression and VSMC adhesion and migration. Expression of the alpha(7)-integrin gene was determined by real-time RT-PCR, whereas protein levels were determined by fluorescence-activated cell sorting analysis. PDGF increased alpha(7) cell surface protein expression (12 and 24 h: 3.3 +/- 0.8- and 3.6 +/- 0.4-fold, P < 0.05 vs. control) and mRNA levels (24 h: 3.1-fold, P < 0.05 vs. control) in a time-dependent manner. Actinomycin D and cycloheximide attenuated PDGF-induced increases in alpha(7)-integrin, indicating the involvement of de novo mRNA and protein synthesis. Treatment with the MAPK inhibitors PD-98059, SP-600125, and SB-203580 attenuated PDGF-induced increases in mRNA. In contrast, PD-98059 and SP-600125, but not SB-203580, attenuated PDGF-induced increases in cell surface protein levels. PDGF-treated VSMC adhered to laminin more efficiently (42 +/- 6% increase, P < 0.01), and this increase was partially inhibited by anti-alpha(7)-integrin function-blocking antibody. However, PDGF did not alter migration on laminin, and there was no effect of the anti-alpha(7)-integrin function-blocking antibody on basal or PDGF-stimulated migration. Immunofluorescence imaging revealed an increase in alpha(7)-integrin distribution along the stress fibers. Together, these observations indicate that PDGF enhances alpha(7)-integrin expression in VSMC and promotes alpha(7)-integrin-mediated adhesion to laminin. | lld:pubmed |
| pubmed-article:16282198 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16282198 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16282198 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16282198 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:16282198 | pubmed:issn | 0363-6143 | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:Martinez-Lemu... | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:MeiningerGera... | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:RamosKenneth... | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:WilsonEmilyE | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:KaufmanStephe... | lld:pubmed |
| pubmed-article:16282198 | pubmed:author | pubmed-author:ChaoJun-TzuJT | lld:pubmed |
| pubmed-article:16282198 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16282198 | pubmed:volume | 290 | lld:pubmed |
| pubmed-article:16282198 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16282198 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16282198 | pubmed:pagination | C972-80 | lld:pubmed |
| pubmed-article:16282198 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:meshHeading | pubmed-meshheading:16282198... | lld:pubmed |
| pubmed-article:16282198 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16282198 | pubmed:articleTitle | Modulation of alpha7-integrin-mediated adhesion and expression by platelet-derived growth factor in vascular smooth muscle cells. | lld:pubmed |
| pubmed-article:16282198 | pubmed:affiliation | Division of Vascular Biology, Cardiovascular Research Institute, The Texas A&M University System Health Science Center, 336 Joe Reynolds Medical Bldg., College Station, 77843, USA. | lld:pubmed |
| pubmed-article:16282198 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16282198 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16282198 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:81008 | entrezgene:pubmed | pubmed-article:16282198 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:16282198 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16282198 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16282198 | lld:pubmed |
