Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2005-11-7
pubmed:abstractText
Group A streptococci (GAS) are responsible for a wide variety of human infections associated with considerable morbidity and mortality. Ever since the first systematic effort by Lancefield to group Streptococcus species by M protein variants, the detection and characterization of Streptococcus by different methods have been an evolving process. The ideal assay for GAS identification not only would provide quick and accurate diagnostic results but also would reveal antibiotic resistance patterns and genotype information, aiding not only in treatment but in epidemiologic assessment as well. The oligonucleotide microarray is a promising new technology which could potentially address this need. In this study, we evaluated the usefulness of oligonucleotide resequencing microarrays for identifying GAS and its associated antibiotic resistance markers. We demonstrated an assay platform that combines the use of resequencing DNA microarrays with either random nucleic acid amplification or multiplex PCR for GAS detection. When detecting Streptococcus pyogenes from coded clinical samples, this approach demonstrated an excellent concordance with a more established culture method. To this end, we showed the potential of resequencing microarrays for efficient and accurate detection of GAS and its associated antibiotic resistance markers with the benefit of sequencing information from microarray analysis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-10221877, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-10325352, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-10582867, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-10885988, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11237843, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11581234, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11691856, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11733479, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11825995, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11880388, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11880392, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11917108, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-11976131, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12030762, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12089254, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12150457, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12239225, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12394813, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12419616, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12429852, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12517855, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12682163, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12821341, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12964112, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-12969551, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-13680391, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-14552738, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-15365022, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-15652219, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-7891551, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-8673920, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-8815115, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-8913465, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-9271035, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-9574721, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-9582189, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-9620418, http://linkedlifedata.com/resource/pubmed/commentcorrection/16272506-9854063
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5690-5
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Use of resequencing oligonucleotide microarrays for identification of Streptococcus pyogenes and associated antibiotic resistance determinants.
pubmed:affiliation
Malcolm Grow Medical Center, Andrews Air Force Base, Maryland 20762, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.