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pubmed-article:16187416pubmed:dateCreated2005-9-27lld:pubmed
pubmed-article:16187416pubmed:abstractTextThe origin of 5-HMF (5-hydroxymethyl-2-furaldehyde) in a Shengmaiyin decoction was investigated by the RP-HPLC method below. A C(18) column (250 mm x 4.6 mm, i.d. 5 microm) with a column temperature of 25 degrees C was used. The mobile phase was a mixture of ultra-pure water-acetonitrile (95:5, V/V) and the flow rate was 1.0 ml/min. The detection wavelength was 280 nm. The injection volume was 1 microl and the running time was about 20 min. The addition of Schisandra was regulated to assess the contribution of an acid environment to the production of 5-HMF. In order to confirm the role of saccharides in the production of 5-HMF, different amount of fructose was used. The 5-HMF level in decoctions of processed and unprocessed Schisandra was investigated in order to determine the origin of 5-HMF. The results showed that 5-HMF was derived mainly from the decoction of Schisandra only and not the mixed decoction of Ophionpogon and Schisandra. The appearance of 5-HMF is not simply the result of the decomposition of saccharides under the acid environment created by Schisandra, but the processing procedure plays an important role in the production of 5-HMF.lld:pubmed
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pubmed-article:16187416pubmed:authorpubmed-author:LiYing-HuaYHlld:pubmed
pubmed-article:16187416pubmed:authorpubmed-author:LuXiu-YangXYlld:pubmed
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pubmed-article:16187416pubmed:pagination1015-21lld:pubmed
pubmed-article:16187416pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:16187416pubmed:year2005lld:pubmed
pubmed-article:16187416pubmed:articleTitleInvestigation on the origin of 5-HMF in Shengmaiyin decoction by RP-HPLC method.lld:pubmed
pubmed-article:16187416pubmed:affiliationInstitute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China.lld:pubmed
pubmed-article:16187416pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16187416pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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