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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2005-9-12
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pubmed:abstractText |
Two plasmid DNA constructs were obtained by cloning separately into the eukaryotic expression vector pcDNA3.1/V5-His-TOPO the wall-associated protein A (wapA) gene of Streptococcus mutans GS-5 or its truncated derivative antigen A (agA) gene encoding a known candidate antigen for dental caries vaccine. The immunogenicity of the two constructs, designated pcDNA-wapA and pcDNA-agA, was compared by intranasal immunization of two groups of mice using the cationic DMRIE-C (1,2-dimyristyloxypropyl-3-dimethylhydroxy ethyl ammonium bromide-cholesterol) as an adjuvant. Immunization with pcDNA-wapA or pcDNA- agA resulted in specific salivary IgA and systemic IgG antibodies to the target antigens after two doses given at 3-week intervals. Higher salivary IgA level was observed in the mice immunized with the pcDNA-wapA vaccine compared to those immunized with the pcDNA-agA vaccine. Furthermore, anti-WapA antibody inhibited S. mutans sucrose-dependent adherence suggesting a potential protection against S. mutans colonization of the tooth, while anti-AgA had no significant effect. Indeed, prediction and analysis of protein epitopes showed that WapA contains highly promiscuous MHC-II binding motifs in addition to those found in AgA. Immunodot assay confirmed that WapA bound biotin-labeled dextran, whereas AgA did not. These data indicated that full-length WapA is a better candidate vaccine antigen than the soluble AgA, which is truncated in the hydrophobic membrane and wall-spanning region.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/(3-dimyristyloxypropyl)(dimethyl)(hy...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Anti-Idiotypic,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/Dextrans,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Quaternary Ammonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/wapA protein, Bacillus subtilis
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1044-5498
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
574-81
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:16153158-Animals,
pubmed-meshheading:16153158-Antibodies, Anti-Idiotypic,
pubmed-meshheading:16153158-Antigens, Bacterial,
pubmed-meshheading:16153158-Bacterial Adhesion,
pubmed-meshheading:16153158-Bacterial Proteins,
pubmed-meshheading:16153158-Biotin,
pubmed-meshheading:16153158-Dental Caries,
pubmed-meshheading:16153158-Dextrans,
pubmed-meshheading:16153158-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16153158-Epitopes,
pubmed-meshheading:16153158-Escherichia coli,
pubmed-meshheading:16153158-Female,
pubmed-meshheading:16153158-Genes, MHC Class II,
pubmed-meshheading:16153158-Immunoglobulin A,
pubmed-meshheading:16153158-Immunoglobulin G,
pubmed-meshheading:16153158-Lipids,
pubmed-meshheading:16153158-Mice,
pubmed-meshheading:16153158-Mice, Inbred BALB C,
pubmed-meshheading:16153158-Plasmids,
pubmed-meshheading:16153158-Quaternary Ammonium Compounds,
pubmed-meshheading:16153158-Saliva,
pubmed-meshheading:16153158-Vaccines, DNA
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pubmed:year |
2005
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pubmed:articleTitle |
Differential immunogenicity of a DNA vaccine containing the Streptococcus mutans wall-associated protein A gene versus that containing a truncated derivative antigen A lacking in the hydrophobic carboxyterminal region.
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pubmed:affiliation |
Department of Biology, University of South Florida, Tampa, 33620, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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