Linked Life Data

16144835

Source:http://linkedlifedata.com/resource/pubmed/id/16144835

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2005-11-7
pubmed:abstractText
The Forkhead Box f1 (Foxf1) transcription factor (previously known as HFH-8 or Freac-1) is expressed in the septum transversum and splanchnic (visceral) mesoderm and is required for proper development of gut-derived organs. Sequence comparisons of mouse and human Foxf1 genes have revealed highly conserved DNA sequences located within the -5.3-kb Foxf1 promoter region and the 400-nucleotide regulatory element located 1 kb 3' to the Foxf1 gene (3'RE). To examine their transcriptional activity during mouse embryonic development, we generated transgenic mice in which the expression of the beta-galactosidase transgene was controlled by the -2.7-kb Foxf1 promoter region, the -5.3-kb Foxf1 promoter region, or the -5.3-kb Foxf1 promoter region fused to the 3'RE. The -5.3-kb Foxf1 promoter sequences induced appropriate transgene expression in the midgut and developing intestine, whereas the -2.7-kb Foxf1 promoter region was transcriptionally inactive. Addition of 3'RE to the -5.3-kb Foxf1 promoter restored proper transgene expression in the foregut, liver, and lung mesenchyme and prevented ectopic transgene expression in the developing nervous system. Cotransfection studies demonstrated that FoxA2 protein bound to the 3'RE region (+4506/+4529 bp) and was sufficient to inhibit expression of the -5.3-kb Foxf1 promoter. Furthermore, C/EBPbeta and HNF-6 proteins bound to the 3'RE region (+4647/+4694 bp) and provided synergistic transcriptional activation of the -5.3-kb Foxf1 promoter in cotransfection assays. These studies demonstrated that the conserved Foxf1 3'RE region is essential for proper tissue-specific regulation of the Foxf1 promoter region during mouse embryogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37908-16
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16144835-Animals, pubmed-meshheading:16144835-Base Sequence, pubmed-meshheading:16144835-Binding Sites, pubmed-meshheading:16144835-Brain, pubmed-meshheading:16144835-CCAAT-Enhancer-Binding Protein-beta, pubmed-meshheading:16144835-Conserved Sequence, pubmed-meshheading:16144835-Evolution, Molecular, pubmed-meshheading:16144835-Forkhead Transcription Factors, pubmed-meshheading:16144835-Gastrointestinal Tract, pubmed-meshheading:16144835-Gene Expression Regulation, Developmental, pubmed-meshheading:16144835-Hepatocyte Nuclear Factor 6, pubmed-meshheading:16144835-Humans, pubmed-meshheading:16144835-Liver, pubmed-meshheading:16144835-Lung, pubmed-meshheading:16144835-Mice, pubmed-meshheading:16144835-Mice, Transgenic, pubmed-meshheading:16144835-Organ Specificity, pubmed-meshheading:16144835-Promoter Regions, Genetic, pubmed-meshheading:16144835-Protein Binding, pubmed-meshheading:16144835-Sequence Homology, Nucleic Acid, pubmed-meshheading:16144835-Spinal Cord, pubmed-meshheading:16144835-beta-Galactosidase
pubmed:year
2005
pubmed:articleTitle
Functional characterization of evolutionarily conserved DNA regions in forkhead box f1 gene locus.
pubmed:affiliation
Department of Medicine, the University of Chicago, Illinois 60637, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural