Linked Life Data

16142356

Source:http://linkedlifedata.com/resource/pubmed/id/16142356

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-9-5
pubmed:abstractText
Death-associated protein kinase (DAPK) was originally identified as a positive mediator of interferon-gamma (IFNgamma)-induced apoptosis in cervical cancer cells, and interferons have been reported to enhance radiosensitivity in various types of squamous cell carcinoma. To examine whether DAPK can regulate cancer cell radiosensitivity, we investigated DAPK expression and radiosensitivity in human cancer cell lines, including the cervical squamous cell carcinoma cell line, ME180, which is both radiosensitive and IFNgamma-sensitive. Of the 5 human cancer cell lines examined, ME180 cells were the most radiosensitive, but their level of DAPK protein expression was undetectable by western blotting. A comparative study of ME180 cells with 2 other uterine cancer cell lines, HHUA and HOKUG, revealed no significant relationships between cellular radiosensitivity and DAPK protein expression or hypermethylation of the DAPK promoter CpG island. INFgamma dose-dependently inhibited ME180 cell proliferation, but did not induce any cell death. IFNgamma significantly enhanced the radiosensitivity of ME180 cells with a slight increase in DAPK protein expression, while irradiation significantly reduced their sensitivity to the growth-inhibitory signals of INFgamma. Analyses of 6 monoclonal cisplatin-resistant subclones established from ME180 cells revealed that all 6 were significantly more radioresistant than the parent ME180 cells without any change in the DAPK protein expression. These results indicate that DAPK does not regulate radiation-induced cell death and that it cannot be either a target molecule for radiotherapy with gene therapy or a prognostic marker for cervical cancer patients treated with radiotherapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1021-335X
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
949-55
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16142356-Antineoplastic Agents, pubmed-meshheading:16142356-Apoptosis, pubmed-meshheading:16142356-Apoptosis Regulatory Proteins, pubmed-meshheading:16142356-Blotting, Western, pubmed-meshheading:16142356-Calcium-Calmodulin-Dependent Protein Kinases, pubmed-meshheading:16142356-Carcinoma, Squamous Cell, pubmed-meshheading:16142356-Cell Line, Tumor, pubmed-meshheading:16142356-Cell Survival, pubmed-meshheading:16142356-Cisplatin, pubmed-meshheading:16142356-CpG Islands, pubmed-meshheading:16142356-DNA, pubmed-meshheading:16142356-DNA Methylation, pubmed-meshheading:16142356-Dose-Response Relationship, Radiation, pubmed-meshheading:16142356-Electrophoresis, Agar Gel, pubmed-meshheading:16142356-Female, pubmed-meshheading:16142356-HeLa Cells, pubmed-meshheading:16142356-Humans, pubmed-meshheading:16142356-Interferon-gamma, pubmed-meshheading:16142356-Mass Spectrometry, pubmed-meshheading:16142356-Polymerase Chain Reaction, pubmed-meshheading:16142356-Promoter Regions, Genetic, pubmed-meshheading:16142356-Radiation, pubmed-meshheading:16142356-Radiation Tolerance, pubmed-meshheading:16142356-Sulfites, pubmed-meshheading:16142356-Uterine Cervical Neoplasms
pubmed:year
2005
pubmed:articleTitle
Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells.
pubmed:affiliation
Department of Obstetrics and Gynecology, Wakayama Medical University, 811-1 Kimi-idera, Wakayama 641-0012, Japan. tetanaka@wakayama-med.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't