Linked Life Data

16079255

Source:http://linkedlifedata.com/resource/pubmed/id/16079255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-8-4
pubmed:abstractText
We have previously reported that bradykinin (BK) represents an influential mitogenic agent in normal breast glandular tissue. We here investigated the mitogenic effects and the signalling pathways of BK in primary cultured human epithelial breast cells obtained from a tumour and from the histologically proven non-malignant tissue adjacent to the tumour. BK provoked cell proliferation, increase in cytosolic calcium, activation of protein kinase C (PKC)-alpha, -beta, -delta, -epsilon and -eta and phosphorylation of the extracellular-regulated kinases 1 and 2 (ERK1/2). The following compounds blocked the proliferative effects of BK: Hyp3-BK, a B2 receptor subtype inhibitor; U73122, a phospholipase C-beta inhibitor; GF109203X, a protein kinase C (PKC) inhibitor; and PD98059, a mitogen-activated protein kinase kinase inhibitor. Gö6976, a Ca(2+)-dependent PKC inhibitor, did not have any effect. In conclusion, the mitogenic effects of BK are retained in peritumour and tumour cells; hence, it is likely that BK has an important role in cancer endorsement and progression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-0795
pubmed:author
pubmed:issnType
Print
pubmed:volume
186
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
291-301
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16079255-Analysis of Variance, pubmed-meshheading:16079255-Bradykinin, pubmed-meshheading:16079255-Breast Neoplasms, pubmed-meshheading:16079255-Calcium, pubmed-meshheading:16079255-Cell Proliferation, pubmed-meshheading:16079255-Enzyme Activation, pubmed-meshheading:16079255-Female, pubmed-meshheading:16079255-Humans, pubmed-meshheading:16079255-Immunoblotting, pubmed-meshheading:16079255-Intracellular Fluid, pubmed-meshheading:16079255-Mitogen-Activated Protein Kinases, pubmed-meshheading:16079255-Mitogens, pubmed-meshheading:16079255-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:16079255-Phosphatidylinositol 3-Kinases, pubmed-meshheading:16079255-Receptors, Bradykinin, pubmed-meshheading:16079255-Signal Transduction, pubmed-meshheading:16079255-Tumor Cells, Cultured
pubmed:year
2005
pubmed:articleTitle
Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture.
pubmed:affiliation
Department of Biological and Environmental Sciences and Technologies, Ecotekne, Università di Lecce, Monteroni, Via Provinciale per Monteroni, 73100 Lecce, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't