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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7050
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pubmed:dateCreated |
2005-7-28
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pubmed:abstractText |
Chemical synapses are complex structures that mediate rapid intercellular signalling in the nervous system. Proteomic studies suggest that several hundred proteins will be found at synaptic specializations. Here we describe a systematic screen to identify genes required for the function or development of Caenorhabditis elegans neuromuscular junctions. A total of 185 genes were identified in an RNA interference screen for decreased acetylcholine secretion; 132 of these genes had not previously been implicated in synaptic transmission. Functional profiles for these genes were determined by comparing secretion defects observed after RNA interference under a variety of conditions. Hierarchical clustering identified groups of functionally related genes, including those involved in the synaptic vesicle cycle, neuropeptide signalling and responsiveness to phorbol esters. Twenty-four genes encoded proteins that were localized to presynaptic specializations. Loss-of-function mutations in 12 genes caused defects in presynaptic structure.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1476-4687
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pubmed:author |
pubmed-author:Ch'ngQueeLimQ,
pubmed-author:DupuyDenisD,
pubmed-author:DybbsMichaelM,
pubmed-author:HillDavid EDE,
pubmed-author:KaplanJoshua MJM,
pubmed-author:KennedyScottS,
pubmed-author:RualJean-FrançoisJF,
pubmed-author:RuvkunGaryG,
pubmed-author:SieburthDerekD,
pubmed-author:TavazoieMasoudM,
pubmed-author:VidalMarcM,
pubmed-author:WangDuoD
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pubmed:issnType |
Electronic
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pubmed:day |
28
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pubmed:volume |
436
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
510-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16049479-Aldicarb,
pubmed-meshheading:16049479-Animals,
pubmed-meshheading:16049479-Caenorhabditis elegans,
pubmed-meshheading:16049479-Caenorhabditis elegans Proteins,
pubmed-meshheading:16049479-Cluster Analysis,
pubmed-meshheading:16049479-Cytoskeleton,
pubmed-meshheading:16049479-Drug Resistance,
pubmed-meshheading:16049479-Fluorescence,
pubmed-meshheading:16049479-Gene Expression Profiling,
pubmed-meshheading:16049479-Membrane Proteins,
pubmed-meshheading:16049479-Microfilament Proteins,
pubmed-meshheading:16049479-Motor Neurons,
pubmed-meshheading:16049479-Mutation,
pubmed-meshheading:16049479-Nerve Tissue Proteins,
pubmed-meshheading:16049479-Neuromuscular Junction,
pubmed-meshheading:16049479-Neuropeptides,
pubmed-meshheading:16049479-Phorbol Esters,
pubmed-meshheading:16049479-Protein Transport,
pubmed-meshheading:16049479-R-SNARE Proteins,
pubmed-meshheading:16049479-RNA Interference,
pubmed-meshheading:16049479-Synapses,
pubmed-meshheading:16049479-Synaptic Transmission,
pubmed-meshheading:16049479-Synaptic Vesicles
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pubmed:year |
2005
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pubmed:articleTitle |
Systematic analysis of genes required for synapse structure and function.
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pubmed:affiliation |
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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