Linked Life Data

1601346

Source:http://linkedlifedata.com/resource/pubmed/id/1601346

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-7-10
pubmed:abstractText
Sex determination by polymerase chain reaction (PCR) analysis of the X-Y homologous amelogenin gene is highly reliable since the detection of an X-specific amplified fragment validates the procedure. Previously, we reported that 250 ng of template DNA are required for sex determination by this method. We report here a refinement of the technique to include dual PCR. Dual PCR using two sets of primers results in the detection of X- and Y-specific amplified fragments from as little as 0.005 ng of template DNA. This is a powerful technique for the analysis of trace forensic samples and its application is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0379-0738
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:geneSymbol
AMXY
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
143-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene.
pubmed:affiliation
Department of Legal Medicine, Shimane Medical University, Izumo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't