Source:http://linkedlifedata.com/resource/pubmed/id/15978570
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0013935,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0021920,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0033413,
umls-concept:C0038250,
umls-concept:C0185117,
umls-concept:C0242767,
umls-concept:C0851285,
umls-concept:C1186763,
umls-concept:C1416447,
umls-concept:C2911684
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| pubmed:issue |
2
|
| pubmed:dateCreated |
2005-7-12
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| pubmed:abstractText |
The s-SHIP protein is a shorter isoform of the longer SHIP1 protein and lacks the N-terminal SH2 domain region contained in SHIP1. s-SHIP is expressed in ES cells and in enriched bone marrow stem cells, and may be controlled by a promoter within intron 5 of the ship1 gene. We therefore examined the potential specificity of promoter activity in ES cells of an intron 5/intron 6 ship1 genomic segment and its tissue specificity within transgenic mice expressing GFP from this promoter region. The results indicate that s-SHIP promoter activity is specific for ES cells in vitro and for known and presumptive stem/progenitor cells throughout embryo development of the transgenic mice. Specific GFP expression was observed in the blastocyst, primordial germ cells, thymus, arterioles, osteoblasts, and skin epidermis. The epidermis/epithelium is the progenitor for hair follicles, mammary tissue, and prostate. Interestingly, each of these latter tissues acquired a few GFP-positive cells in the course of their development from the epithelial layers, and these cells express marker proteins for stem/progenitor cells. These results identify potential stem cell populations, mark these cells for analyses in normal and cancer development, and implicate s-SHIP as an important protein in stem/progenitor cell function.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0012-1606
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
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| pubmed:volume |
283
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
503-21
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15978570-Animals,
pubmed-meshheading:15978570-Blastocyst,
pubmed-meshheading:15978570-Cell Line,
pubmed-meshheading:15978570-Embryo, Mammalian,
pubmed-meshheading:15978570-Epidermis,
pubmed-meshheading:15978570-Epithelial Cells,
pubmed-meshheading:15978570-Female,
pubmed-meshheading:15978570-Gene Expression Regulation, Developmental,
pubmed-meshheading:15978570-Germ Cells,
pubmed-meshheading:15978570-Green Fluorescent Proteins,
pubmed-meshheading:15978570-Introns,
pubmed-meshheading:15978570-Male,
pubmed-meshheading:15978570-Mice,
pubmed-meshheading:15978570-Mice, Inbred C57BL,
pubmed-meshheading:15978570-Mice, Inbred CBA,
pubmed-meshheading:15978570-Mice, Transgenic,
pubmed-meshheading:15978570-Organ Specificity,
pubmed-meshheading:15978570-Phosphoric Monoester Hydrolases,
pubmed-meshheading:15978570-Promoter Regions, Genetic,
pubmed-meshheading:15978570-Stem Cells,
pubmed-meshheading:15978570-Transgenes
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| pubmed:year |
2005
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| pubmed:articleTitle |
The intron 5/6 promoter region of the ship1 gene regulates expression in stem/progenitor cells of the mouse embryo.
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| pubmed:affiliation |
Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA. lrohrsch@fhcrc.org
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
|