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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2005-6-23
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pubmed:abstractText |
The transcription factor nuclear factor-kappaB (NF-kappaB) regulates cell survival pathways, but the molecular mechanisms involved are not completely understood. Here, we developed a NF-kappaB reporter cell system derived from CEM T leukemic cells to monitor the consequences of NF-kappaB activation following DNA damage insults. Cells that activated NF-kappaB in response to ionizing radiation or etoposide arrested in the G2-M phase for a prolonged time, which was followed by increased cell cycle reentry and survival. In contrast, those that failed to activate NF-kappaB underwent transient G2-M arrest and extensive cell death. Importantly, p21waf1/cip1 was induced in S-G2-M phases in a NF-kappaB-dependent manner, and RNA interference of this cell cycle regulator reduced the observed NF-kappaB-dependent phenotypes. Thus, cell cycle-coupled induction of p21waf1/cip1 by NF-kappaB represents a resistance mechanism in certain cancer cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Camptothecin,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Doxorubicin,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Etoposide,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1541-7786
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
345-53
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:15972853-Antibiotics, Antineoplastic,
pubmed-meshheading:15972853-Apoptosis,
pubmed-meshheading:15972853-Breast Neoplasms,
pubmed-meshheading:15972853-Camptothecin,
pubmed-meshheading:15972853-Cell Cycle Proteins,
pubmed-meshheading:15972853-Cell Line, Tumor,
pubmed-meshheading:15972853-Cell Survival,
pubmed-meshheading:15972853-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:15972853-Doxorubicin,
pubmed-meshheading:15972853-Enzyme Inhibitors,
pubmed-meshheading:15972853-Etoposide,
pubmed-meshheading:15972853-Female,
pubmed-meshheading:15972853-Flow Cytometry,
pubmed-meshheading:15972853-G2 Phase,
pubmed-meshheading:15972853-Humans,
pubmed-meshheading:15972853-Mitosis,
pubmed-meshheading:15972853-NF-kappa B,
pubmed-meshheading:15972853-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:15972853-RNA Interference,
pubmed-meshheading:15972853-Radiation, Ionizing,
pubmed-meshheading:15972853-T-Lymphocytes,
pubmed-meshheading:15972853-Time Factors,
pubmed-meshheading:15972853-Tumor Necrosis Factor-alpha
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pubmed:year |
2005
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pubmed:articleTitle |
Enhanced G2-M arrest by nuclear factor-{kappa}B-dependent p21waf1/cip1 induction.
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pubmed:affiliation |
Department of Pharmacology, University of Wisconsin, 301 Service Memorial Institute, 1300 University Avenue, Madison, WI 53706, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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