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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1980-1-19
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pubmed:abstractText |
Specialized transducing phages containing the thr-trpR region of the Escherichia coli chromosome were derived from a strain with lambda prophage inserted in thr. Cloning of segments of the chromosomal deoxyribonucleic acid of one such lambda thr + trpR+ phage in various plasmid vectors established that a 1.3-kilobase BamHI fragment carried trpR+ intact. Strains with a multicopy plasmid vector containing the BamHI insert produced 20-fold-higher levels of trp aporepressor than did the wild-type strain of Escherichia coli. Similarly, induction of lambda thr + trpR+ lysogens resulted in increased aporepressor levels. The 1.3-kilobase trpR+ BamHI fragment was inserted in either orientation downstream from lambda pLN in a plasmid vector in which transcription from lambda pL was under the control of a temperature-sensitive lambda repressor. Induction established the orientation of transcription of trpR and led to the production of 100-fold-increased levels of trp aporepressor. A presumptive 23,500-dalton trpR+ polypeptide was detected by using lambda pLNtrpR+ plasmid deoxyribonucleic acid in a cell-free transcription-translation system.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-1052323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-1102702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-13475269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-13671770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-318646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-330867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-340930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-346574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-351194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-368800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-372049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4338582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4358553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4366077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4390186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4528605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4560950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4582891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4582893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4583233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4589345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4601822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4610576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4897801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-4940311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-673857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-770450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-773363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-775105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-781293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-845942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/159284-909783
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
106-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:159284-Bacterial Proteins,
pubmed-meshheading:159284-Bacteriophage lambda,
pubmed-meshheading:159284-Cloning, Molecular,
pubmed-meshheading:159284-DNA, Bacterial,
pubmed-meshheading:159284-DNA, Recombinant,
pubmed-meshheading:159284-Escherichia coli,
pubmed-meshheading:159284-Genes, Regulator,
pubmed-meshheading:159284-Plasmids,
pubmed-meshheading:159284-Repressor Proteins,
pubmed-meshheading:159284-Transcription, Genetic,
pubmed-meshheading:159284-Transcription Factors,
pubmed-meshheading:159284-Tryptophan
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pubmed:year |
1979
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pubmed:articleTitle |
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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