| pubmed-article:15912553 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0205431 | lld:lifeskim |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0034284 | lld:lifeskim |
| pubmed-article:15912553 | lifeskim:mentions | umls-concept:C0896246 | lld:lifeskim |
| pubmed-article:15912553 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:15912553 | pubmed:dateCreated | 2005-7-4 | lld:pubmed |
| pubmed-article:15912553 | pubmed:abstractText | The ability of cationic phosphoramidate pyrimidine alpha-oligonucleotides (ONs) to form triplexes with DNA duplexes was investigated by UV melting experiments, circular dichroism spectroscopy and gel mobility shift experiments. Replacement of the phosphodiester linkages in alpha-ONs with positively charged phosphoramidate linkages results in more efficient triplex formation, the triplex stability increasing with the number of positive charges. At a neutral pH and in the absence of magnesium ions, it was found that a fully cationic phosphoramidate alpha-TFO (triplex-forming oligonucleotide) forms a highly stable triplex that melts at a higher temperature than the duplex target. No hysteresis between the annealing and melting curves was noticed; this indicates fast association. Moreover, the recognition of a DNA duplex with a cationic alpha-TFO through Hoogsteen base pairing is highly sequence-specific. To the best of our knowledge, this is the first report of stable triplexes in the pyrimidine motif formed by cationic alpha-oligonucleotides and duplex targets. | lld:pubmed |
| pubmed-article:15912553 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15912553 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15912553 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15912553 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15912553 | pubmed:issn | 1439-4227 | lld:pubmed |
| pubmed-article:15912553 | pubmed:author | pubmed-author:HeitzFrédéric... | lld:pubmed |
| pubmed-article:15912553 | pubmed:author | pubmed-author:VasseurJean-J... | lld:pubmed |
| pubmed-article:15912553 | pubmed:author | pubmed-author:DebartFrançoi... | lld:pubmed |
| pubmed-article:15912553 | pubmed:author | pubmed-author:MichelThibaut... | lld:pubmed |
| pubmed-article:15912553 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15912553 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:15912553 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15912553 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15912553 | pubmed:pagination | 1254-62 | lld:pubmed |
| pubmed-article:15912553 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
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| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:meshHeading | pubmed-meshheading:15912553... | lld:pubmed |
| pubmed-article:15912553 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15912553 | pubmed:articleTitle | Highly stable DNA triplexes formed with cationic phosphoramidate pyrimidine alpha-oligonucleotides. | lld:pubmed |
| pubmed-article:15912553 | pubmed:affiliation | LCOBS, UMR 5625 CNRS-UMII, CC 008, Université Montpellier II, Place Eugène Bataillon, 34095 Montpellier Cedex 05, France. | lld:pubmed |
| pubmed-article:15912553 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15912553 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15912553 | lld:pubmed |
