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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2005-5-4
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pubmed:abstractText |
Clostridium acetobutylicum ATCC 824 was selected for the homologous overexpression of its Fe-only hydrogenase and for the heterologous expressions of the Chlamydomonas reinhardtii and Scenedesmus obliquus HydA1 Fe-only hydrogenases. The three Strep tag II-tagged Fe-only hydrogenases were isolated with high specific activities by two-step column chromatography. The purified algal hydrogenases evolve hydrogen with rates of around 700 micromol H(2) min(-1) mg(-1), while HydA from C. acetobutylicum (HydA(Ca)) shows the highest activity (5,522 micromol H(2) min(-1) mg(-1)) in the direction of hydrogen uptake. Further, kinetic parameters and substrate specificity were reported. An electron paramagnetic resonance (EPR) analysis of the thionin-oxidized HydA(Ca) protein indicates a characteristic rhombic EPR signal that is typical for the oxidized H cluster of Fe-only hydrogenases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-10368269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-10669801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-10684972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-10858294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-11096090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-11400057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-11466286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-11524134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-11846805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-12620841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-12823545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-12902297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-15082711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-2173950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-2822711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-3015247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-3028789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-7867955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-8073038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-8113186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-8386500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-8513797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-8626337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-9606145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15870373-9836629
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2777-81
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
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pubmed:year |
2005
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pubmed:articleTitle |
Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities.
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pubmed:affiliation |
Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA, 135 Avenue de Rangueil, 31077 Toulouse Cédex 4, France. girbal@insa-toulouse.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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