Source:http://linkedlifedata.com/resource/pubmed/id/15860221
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-4-29
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| pubmed:abstractText |
Impaired immune reconstitution following allogeneic T-cell depleted bone marrow transplantation (allo-TCD-BMT) is a major obstacle to its clinical application. Stromal cell line QXMSC1, established from bone marrow cells of BALB/c(H-2d), was transfected with murine IL-3 and/ or IL-2 gene, and injected into lethally irradiated C57BL/6(H2b) mice. We evaluated its effects on immunologic and hematopoietic reconstitution after allo-TCD-BMT. The results showed that QXMSC1-IL-3 + IL-2 could significantly increase the numbers of hematopoietic primitive progenitors (CFU-S), committed progenitors (CFU-GM, and BFU-E), and lymphocytes (CD8+ cells, CD4+ cells, and B cells). Similarly, immune functions of recipient mice were significantly enhanced in the QXMSC1-IL-3 + IL-2 group. In addition, QXMSC1-IL-3 or QXMSC1-IL-2 also exerted apparent effects on accelerating immune reconstitution, but these effects were far less than that of QXMSC1-IL-3 + IL-2. Our results demonstrated that stromal cell-mediated IL-3 and IL-2 gene therapy may be a potent approach in promoting immunologic and hematopoietic reconstitution after allo-TCD-BMT.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
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| pubmed:issn |
0165-2478
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
98
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
216-24
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| pubmed:meshHeading |
pubmed-meshheading:15860221-Animals,
pubmed-meshheading:15860221-Bone Marrow Transplantation,
pubmed-meshheading:15860221-Cell Differentiation,
pubmed-meshheading:15860221-Cell Line,
pubmed-meshheading:15860221-Cell Proliferation,
pubmed-meshheading:15860221-Cytokines,
pubmed-meshheading:15860221-Gene Expression,
pubmed-meshheading:15860221-Gene Therapy,
pubmed-meshheading:15860221-Genetic Engineering,
pubmed-meshheading:15860221-Hematopoiesis,
pubmed-meshheading:15860221-Hematopoietic Stem Cells,
pubmed-meshheading:15860221-Interleukin-2,
pubmed-meshheading:15860221-Interleukin-3,
pubmed-meshheading:15860221-Leukocyte Count,
pubmed-meshheading:15860221-Mice,
pubmed-meshheading:15860221-Mice, Inbred BALB C,
pubmed-meshheading:15860221-Mice, Inbred C57BL,
pubmed-meshheading:15860221-Spleen,
pubmed-meshheading:15860221-Stromal Cells,
pubmed-meshheading:15860221-Transplantation, Homologous
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Cytokines transduced bone marrow stromal cell lines promote immunohematopoietic reconstitution in mice after allogeneic bone marrow transplantation.
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| pubmed:affiliation |
Department of Immunology, Peking University Health Science Center, Beijing, China.
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| pubmed:publicationType |
Journal Article
|