Linked Life Data

15840698

Source:http://linkedlifedata.com/resource/pubmed/id/15840698

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2005-7-21
pubmed:abstractText
The normal counterpart of the neoplastic B cells in Burkitt lymphoma (BL) is still unclear. Based on immunoglobulin gene rearrangement studies, some authors suggest an origin from germinal center cells and others from memory B cells. However, most of these studies rely on cell lines or on a small series of cases. To help clarify the cell of origin of BL, semi-nested polymerase chain reaction (PCR) was performed to amplify the VDJ rearrangements of the immunoglobulin heavy chain (V(H)) genes, and the resultant amplificates were sequenced for comparison with known germline V(H) segments. The results of this approach revealed that all cases (15 endemic BL [eBL], 10 sporadic BL [sBL], and 6 AIDS-related BL) harbor mutated V(H) genes, with different mutation ranges among the 3 types of BL. The eBL and AIDS-related forms showed considerably higher mutation rates than the sBL form (5.1%, 5.4%, and 1.5%, respectively). The mutations in eBL and AIDS-related BL also showed signs of antigen selection, whereas no signs of antigen selection were found in sBL. Finally, after subcloning the amplificates, sequence analysis revealed no signs of ongoing mutations in any of the cases analyzed. Given that one of the main differences between eBL and AIDS-related BL on the one hand and sBL on the other hand is the association with Epstein-Barr virus (EBV), we compared EBV-positive and EBV-negative BLs independently of their geographic origin and HIV status. The differences in the number of somatic mutations and antigen selection were even more evident when this approach was used. According to our molecular results, it appears that EBV-positive and EBV-negative BL may originate from 2 distinct subsets of B cells, pointing to a particular role for the germinal-center reaction in the pathogenesis of these tumors. The different types of C-MYC translocation reported in BL may also be related to the different stages of B-cell maturation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1031-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15840698-Adolescent, pubmed-meshheading:15840698-Adult, pubmed-meshheading:15840698-Aged, pubmed-meshheading:15840698-B-Lymphocytes, pubmed-meshheading:15840698-Burkitt Lymphoma, pubmed-meshheading:15840698-Child, pubmed-meshheading:15840698-Child, Preschool, pubmed-meshheading:15840698-Gene Rearrangement, pubmed-meshheading:15840698-Genes, Immunoglobulin, pubmed-meshheading:15840698-Germinal Center, pubmed-meshheading:15840698-Herpesvirus 4, Human, pubmed-meshheading:15840698-Humans, pubmed-meshheading:15840698-Immunoglobulin Heavy Chains, pubmed-meshheading:15840698-Lymphoma, AIDS-Related, pubmed-meshheading:15840698-Middle Aged, pubmed-meshheading:15840698-Mutation, pubmed-meshheading:15840698-Polymerase Chain Reaction, pubmed-meshheading:15840698-Sequence Analysis, DNA
pubmed:year
2005
pubmed:articleTitle
Immunoglobulin gene analysis reveals 2 distinct cells of origin for EBV-positive and EBV-negative Burkitt lymphomas.
pubmed:affiliation
Department of Human Pathology and Oncology, University of Siena, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't