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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 7
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pubmed:dateCreated |
2005-6-27
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pubmed:abstractText |
The pro-inflammatory cytokine interleukin-6 (IL-6) together with its soluble receptor (sIL-6R) induces and maintains thermal hyperalgesia. It facilitates the heat-induced release of calcitonin gene-related peptide from rat cutaneous nociceptors in vivo and in vitro. Here we report that exposure of nociceptive neurons to the IL-6-sIL-6R complex or the gp130-stimulating designer IL-6-sIL-6R fusion protein Hyper-IL-6 (HIL-6) resulted in a potentiation of heat-activated inward currents (I(heat)) and a shift of activation thresholds towards lower temperatures without affecting intracellular calcium levels. The Janus tyrosine kinase inhibitor AG490, the selective protein kinase C (PKC) inhibitor, bisindolylmaleimide 1 (BIM1), as well as rottlerin, a selective blocker of the PKCdelta isoform, but not the cyclooxygenase inhibitor indomethacin, effectively reduced the effect. Reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization revealed expression of mRNA for the signal-transducing beta subunit of the receptor gp130 in neuronal somata, rather than satellite cells in rat dorsal root ganglia. Together, the results suggest that IL-6-sIL-6R acts directly on sensory neurons. It increases their susceptibility to noxious heat via the gp130/Jak/PKCdelta signalling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetophenones,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Benzopyrans,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokine Receptor gp130,
http://linkedlifedata.com/resource/pubmed/chemical/Il6st protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Jak1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Maleimides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Prkcd protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-delta,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrphostins,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-cyano-(3,4-dihydroxy)-N-benzyl...,
http://linkedlifedata.com/resource/pubmed/chemical/bisindolylmaleimide,
http://linkedlifedata.com/resource/pubmed/chemical/rottlerin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1460-2156
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1634-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15817518-Acetophenones,
pubmed-meshheading:15817518-Animals,
pubmed-meshheading:15817518-Antigens, CD,
pubmed-meshheading:15817518-Benzopyrans,
pubmed-meshheading:15817518-Calcium,
pubmed-meshheading:15817518-Cells, Cultured,
pubmed-meshheading:15817518-Cyclooxygenase Inhibitors,
pubmed-meshheading:15817518-Cytokine Receptor gp130,
pubmed-meshheading:15817518-Female,
pubmed-meshheading:15817518-Ganglia, Spinal,
pubmed-meshheading:15817518-Hot Temperature,
pubmed-meshheading:15817518-In Situ Hybridization,
pubmed-meshheading:15817518-Indoles,
pubmed-meshheading:15817518-Indomethacin,
pubmed-meshheading:15817518-Interleukin-6,
pubmed-meshheading:15817518-Janus Kinase 1,
pubmed-meshheading:15817518-Maleimides,
pubmed-meshheading:15817518-Membrane Glycoproteins,
pubmed-meshheading:15817518-Neurons, Afferent,
pubmed-meshheading:15817518-Protein Kinase C,
pubmed-meshheading:15817518-Protein Kinase C-delta,
pubmed-meshheading:15817518-Protein-Tyrosine Kinases,
pubmed-meshheading:15817518-RNA, Messenger,
pubmed-meshheading:15817518-Rats,
pubmed-meshheading:15817518-Rats, Wistar,
pubmed-meshheading:15817518-Receptors, Interleukin-6,
pubmed-meshheading:15817518-Recombinant Fusion Proteins,
pubmed-meshheading:15817518-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15817518-Sensory Thresholds,
pubmed-meshheading:15817518-Signal Transduction,
pubmed-meshheading:15817518-Tyrphostins
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pubmed:year |
2005
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pubmed:articleTitle |
Fast modulation of heat-activated ionic current by proinflammatory interleukin 6 in rat sensory neurons.
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pubmed:affiliation |
Institut für Physiologie und Experimentelle Pathophysiologie, Friedrich-Alexander Universität, Erlangen-Nürnberg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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