Linked Life Data

15814528

Source:http://linkedlifedata.com/resource/pubmed/id/15814528

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-7-11
pubmed:abstractText
Cytochrome P-450 (CYP)-dependent epoxyeicosatrienoic acids (EETs) dilate rat preglomerular microvessels when adenosine(2A) receptors (A(2A)R) are stimulated. As high salt (HS) intake increases epoxygenase activity and adenosine levels, we hypothesized that renal adenosine responses would be greater in HS-fed rats. Male Sprague-Dawley rats were fed either HS (4.0% NaCl) or normal salt (NS; 0.4% NaCl) diet. On day 8, isolated kidneys were perfused with Krebs' buffer containing indomethacin (10 microM) and L-NAME (200 microM) and preconstricted to approximately 150 mmHg with infusion of phenylephrine (10(-7) M). Renal effluents were extracted for analysis of eicosanoids by gas chromatography-mass spectrometry. Bolus injections of the stable adenosine analog 2-chloroadenosine (2-CA; 0.1-10 microg) resulted in dose-dependent dilation; at 10 microg, perfusion pressure (PP) was lowered to a greater extent in the kidneys of HS rats compared with NS rats (-60 +/- 4 vs. -31 +/- 8 mmHg; P < 0.05) and the area of response was increased (27 +/- 6 vs. 9 +/- 4 mm(2); P < 0.05), as was EET release (132 +/- 23 vs. 38 +/- 18 ng; P < 0.05). HS treatment increased A(2A)R and CYP2C23 protein expression. A selective epoxygenase inhibitor, MS-PPOH (12 microM), significantly reduced the response to 2-CA in HS rats; PP, area of response, and EET release decreased by 40, 70, and 81%, respectively, whereas lesser changes were evident in NS kidneys. Thus the greater vasodilator response to 2-CA seen in kidneys obtained from HS-fed rats was mediated by increased EET release. As EETs are renal vasodilator and natriuretic eicosanoids, interactions between adenosine and EETs may contribute to the adaptive response to HS intake.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1931-857X
pubmed:author
pubmed:issnType
Print
pubmed:volume
289
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F386-92
pubmed:dateRevised
2011-4-28
pubmed:meshHeading
pubmed-meshheading:15814528-2-Chloroadenosine, pubmed-meshheading:15814528-8,11,14-Eicosatrienoic Acid, pubmed-meshheading:15814528-Adenosine, pubmed-meshheading:15814528-Animals, pubmed-meshheading:15814528-Blotting, Western, pubmed-meshheading:15814528-Chromatography, High Pressure Liquid, pubmed-meshheading:15814528-Dose-Response Relationship, Drug, pubmed-meshheading:15814528-Gas Chromatography-Mass Spectrometry, pubmed-meshheading:15814528-Hydroxyeicosatetraenoic Acids, pubmed-meshheading:15814528-Kidney, pubmed-meshheading:15814528-Male, pubmed-meshheading:15814528-Perfusion, pubmed-meshheading:15814528-Phenethylamines, pubmed-meshheading:15814528-Rats, pubmed-meshheading:15814528-Rats, Sprague-Dawley, pubmed-meshheading:15814528-Receptor, Adenosine A2A, pubmed-meshheading:15814528-Renal Circulation, pubmed-meshheading:15814528-Sodium, Dietary, pubmed-meshheading:15814528-Vasodilation
pubmed:year
2005
pubmed:articleTitle
Exaggerated response to adenosine in kidneys from high salt-fed rats: role of epoxyeicosatrienoic acids.
pubmed:affiliation
Department of Pharmacology, New York Medical College, Valhalla, 10595, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, N.I.H., Extramural