Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017968,
umls-concept:C0085535,
umls-concept:C0178719,
umls-concept:C0181904,
umls-concept:C0282639,
umls-concept:C0336791,
umls-concept:C0542341,
umls-concept:C0596972,
umls-concept:C0728873,
umls-concept:C1442792,
umls-concept:C1511938,
umls-concept:C1521743,
umls-concept:C1704646
|
pubmed:issue |
3
|
pubmed:dateCreated |
1992-6-9
|
pubmed:abstractText |
After treatment with swainsonine, an inhibitor of both lysosomal alpha-mannosidase and Golgi alpha-mannosidase-II activities, analysis of [3H]mannose-labeled glycans showed that HT-29 cells, derived from a human colonic adenocarcinoma, displayed distinct patterns of N-glycan expression, depending upon their state of enterocytic differentiation. In differentiated HT-29 cells hybrid-type chains were detected, whereas undifferentiated HT-29 cells accumulated high-mannose-type oligosaccharide, despite our demonstration of Golgi alpha-mannosidase-II activity in both cell populations. Pulse/chase experiments carried out in the presence of swainsonine revealed that the persistence of high-mannose-type chains in undifferentiated HT-29 cells was the result of the stabilization of glycoproteins substituted with these glycans. These data suggest that in undifferentiated HT-29 cells, glycoproteins with high-mannose-type oligosaccharides are delivered to a degradative compartment containing swainsonine-sensitive alpha-mannosidase(s), whereas in differentiated HT-29 cells glycoproteins enter a compartment in which alpha-mannosidase II (Golgi apparatus) is present. Thus, this apparent dual effect of swainsonine on N-glycan trimming may reflect differences in the intracellular traffic of glycoproteins as a function of the state of enterocytic differentiation of HT-29 cells.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mannosidases,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Swainsonine,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Mannosidase
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0014-2956
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
1
|
pubmed:volume |
205
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1169-74
|
pubmed:dateRevised |
2007-7-23
|
pubmed:meshHeading |
pubmed-meshheading:1577000-Adenocarcinoma,
pubmed-meshheading:1577000-Cell Differentiation,
pubmed-meshheading:1577000-Cell Line,
pubmed-meshheading:1577000-Chromatography, High Pressure Liquid,
pubmed-meshheading:1577000-Chromatography, Liquid,
pubmed-meshheading:1577000-Colonic Neoplasms,
pubmed-meshheading:1577000-Glycoproteins,
pubmed-meshheading:1577000-Golgi Apparatus,
pubmed-meshheading:1577000-Humans,
pubmed-meshheading:1577000-Lysosomes,
pubmed-meshheading:1577000-Mannosidases,
pubmed-meshheading:1577000-Polysaccharides,
pubmed-meshheading:1577000-Swainsonine,
pubmed-meshheading:1577000-Tumor Cells, Cultured,
pubmed-meshheading:1577000-alpha-Mannosidase
|
pubmed:year |
1992
|
pubmed:articleTitle |
Swainsonine is a useful tool to monitor the intracellular traffic of N-linked glycoproteins as a function of the state of enterocytic differentiation of HT-29 cells.
|
pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 180, Université René Descartes, Paris, France.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|