Linked Life Data

15755640

Source:http://linkedlifedata.com/resource/pubmed/id/15755640

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-3-9
pubmed:abstractText
Four kaempferol glycosides were isolated from the leaves of Cinnamomum osmophloeum Kaneh, a Taiwan endemic tree. These compounds namely, kaempferitrin (1), kaempferol 3-O-beta-D-glucopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-7-O-alpha-L-rhamnopyranoside (2), kaempferol 3-O-beta-D-apiofuranosyl-(1-->2)-alpha-L-arabinofuranosyl-7-O-alpha-L-rhamnopyranoside (3), and kaempferol 3-O-beta-D-apiofuranosy-(1-->4)-alpha-L-rhamnopyranosyl-7-O-alpha-L-rhamnopyranoside (4). The structure of compound 2 was determined by spectroscopic analyses and acid hydrolysis. The isolates 1-4 were evaluated as inhibitors of some macrophage functions involved in the inflammatory process. These four compounds inhibited lipopolysaccharide (LPS) and interferon (IFN)-gamma-induced nitric oxide (NO), and cytokines [tumor necrosis factor (TNF)-alpha and interleukin (IL)-12] in a dose-dependent manner. The concentration of 50% inhibition (IC(50)) of NO by compounds 1, 3, 4 were 40, 15, 20microM, respectively. In parallel, these concentrations were approximately in a similar manner to that observed for TNF-alpha and IL-12 production. However, compound 2 inhibited NO and cytokines production by 30% at 100microM concentration. On the other hand, compounds 3 and 4 showed no inhibitory effect on the production of NO from macrophages, when inducible NO synthase was already expressed by the stimulation with LPS and IFN-gamma. Taken together, our results provide evidence that isolates of C. osmophloeum possess an anti-inflammatory potential which constitutes a previously unrecognized biological activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0968-0896
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2381-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15755640-Animals, pubmed-meshheading:15755640-Carbohydrate Conformation, pubmed-meshheading:15755640-Cinnamomum, pubmed-meshheading:15755640-Dose-Response Relationship, Drug, pubmed-meshheading:15755640-Female, pubmed-meshheading:15755640-Flavonols, pubmed-meshheading:15755640-Glycosides, pubmed-meshheading:15755640-Inflammation Mediators, pubmed-meshheading:15755640-Interferon-gamma, pubmed-meshheading:15755640-Interleukin-12, pubmed-meshheading:15755640-Lipopolysaccharides, pubmed-meshheading:15755640-Macrophage Activation, pubmed-meshheading:15755640-Macrophages, pubmed-meshheading:15755640-Mice, pubmed-meshheading:15755640-Mice, Inbred BALB C, pubmed-meshheading:15755640-Molecular Sequence Data, pubmed-meshheading:15755640-Nitric Oxide, pubmed-meshheading:15755640-Nitric Oxide Synthase, pubmed-meshheading:15755640-Plant Leaves, pubmed-meshheading:15755640-Structure-Activity Relationship, pubmed-meshheading:15755640-Tumor Necrosis Factor-alpha
pubmed:year
2005
pubmed:articleTitle
Inhibitory effects of flavonol glycosides from Cinnamomum osmophloeum on inflammatory mediators in LPS/IFN-gamma-activated murine macrophages.
pubmed:affiliation
Department of Microbiology, School of Medicine, China Medical University, Taichung 400, Taiwan, ROC.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't