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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0018787,
umls-concept:C0020792,
umls-concept:C0038250,
umls-concept:C0185117,
umls-concept:C0300423,
umls-concept:C0596508,
umls-concept:C1332729,
umls-concept:C1334306,
umls-concept:C1335205,
umls-concept:C1820288,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
2005-3-4
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pubmed:abstractText |
We evaluated the expression of the FLK1, one of the lateral mesoderm early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1+ cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1- cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1+cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1+ CD31-VE-cadherin-phenotype. Our findings indicate that FLK1+cells, especially FLK1+ CD31-VE-cadherin-cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1530-6860
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
371-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15746180-Antigens, CD,
pubmed-meshheading:15746180-Antigens, CD31,
pubmed-meshheading:15746180-Cadherins,
pubmed-meshheading:15746180-Cell Differentiation,
pubmed-meshheading:15746180-Cell Line,
pubmed-meshheading:15746180-Coculture Techniques,
pubmed-meshheading:15746180-Embryo, Mammalian,
pubmed-meshheading:15746180-Embryo, Nonmammalian,
pubmed-meshheading:15746180-Endothelial Cells,
pubmed-meshheading:15746180-Gene Expression,
pubmed-meshheading:15746180-Green Fluorescent Proteins,
pubmed-meshheading:15746180-Hematopoietic Stem Cells,
pubmed-meshheading:15746180-Immunohistochemistry,
pubmed-meshheading:15746180-Myocardium,
pubmed-meshheading:15746180-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15746180-Stem Cells,
pubmed-meshheading:15746180-Time Factors,
pubmed-meshheading:15746180-Transfection,
pubmed-meshheading:15746180-Vascular Endothelial Growth Factor Receptor-2
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pubmed:year |
2005
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pubmed:articleTitle |
Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation.
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pubmed:affiliation |
Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto city, Kyoto, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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