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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2005-2-15
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pubmed:abstractText |
The aim of this study was to determine the effects of IGF-I on the viability and development of porcine presumptive diploid parthenotes developing in vitro. In vitro derived 2-cell parthenotes were cultured to blastocysts with or without exogenous IGF-I in the presence or absence of BSA. The addition of 10 or 100 ng/ml IGF-I into NCSU 23 medium did not enhance the development of 2-cell parthenotes to the blastocyst stage. However, IGF-I increased cell numbers (P < 0.001) in the presence of BSA. Similarly, IGF-I alone did not affect apoptosis in the blastocysts but when combined with BSA, it reduced apoptosis (P < 0.05). Real time reverse-transcription polymerase chain reaction revealed that IGF-I significantly decreased the mRNA expression (pro-apoptotic gene Fas or Bak and anti-apoptotic gene Bcl-xL) ratios of both Fas/Bcl-xL (P < 0.01) and Bak/Bcl-xL (P < 0.01) in the presence of 0.4% BSA. These data suggest that IGF-I synergies with BSA to act as a mitogen and apoptotic survival factor in porcine preimplantation parthenotes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0093-691X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1070-80
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:15710194-Animals,
pubmed-meshheading:15710194-Antigens, CD95,
pubmed-meshheading:15710194-Apoptosis,
pubmed-meshheading:15710194-Cell Count,
pubmed-meshheading:15710194-Embryo Culture Techniques,
pubmed-meshheading:15710194-Embryonic Development,
pubmed-meshheading:15710194-Gene Expression,
pubmed-meshheading:15710194-In Situ Nick-End Labeling,
pubmed-meshheading:15710194-Insulin-Like Growth Factor I,
pubmed-meshheading:15710194-Membrane Proteins,
pubmed-meshheading:15710194-Microscopy, Confocal,
pubmed-meshheading:15710194-Oocytes,
pubmed-meshheading:15710194-Parthenogenesis,
pubmed-meshheading:15710194-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15710194-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15710194-Serum Albumin, Bovine,
pubmed-meshheading:15710194-Swine,
pubmed-meshheading:15710194-bcl-2 Homologous Antagonist-Killer Protein,
pubmed-meshheading:15710194-bcl-X Protein
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pubmed:year |
2005
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pubmed:articleTitle |
Insulin-like growth factor-I alters apoptosis related genes and reduces apoptosis in porcine parthenotes developing in vitro.
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pubmed:affiliation |
Department of Animal Sciences, Chungbuk National University, Gaesin-dong, Cheongju, Chungbuk, 361-763, South Korea.
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pubmed:publicationType |
Journal Article
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