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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-1-31
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pubmed:abstractText |
Hemoglobin (Hb) was successfully immobilized in dimethyldioctadecyl ammonium bromide (DOAB) film at pyrolytic graphite (PG) electrode. Electrochemical experiments revealed that Hb in DOAB film exhibited a pair of well-defined, quasi-reversible cyclic voltammetric peaks at about -0.160 V versus saturated calomel electrode (SCE) in pH 5.0 buffer, characteristic of the heme Fe(III)/Fe(II) redox couple of Hb. The electron transfer (eT) rate between Hb and the PG electrode was 0.10 s(-1). Positions of the Soret absorbance band indicated that the Hb retained its secondary structure and was similar to its native state. Furthermore, the Hb in DOAB film acted as a biological catalyst towards the reduction of nitric oxide (NO). The voltammetric response of NO at the Hb-DOAB modified electrode could be used to determine the concentration of NO in solution.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0165-022X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
143-51
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15680284-Biosensing Techniques,
pubmed-meshheading:15680284-Catalysis,
pubmed-meshheading:15680284-Electrochemistry,
pubmed-meshheading:15680284-Electrodes,
pubmed-meshheading:15680284-Electrons,
pubmed-meshheading:15680284-Heme,
pubmed-meshheading:15680284-Hemoglobins,
pubmed-meshheading:15680284-Humans,
pubmed-meshheading:15680284-Hydrogen-Ion Concentration,
pubmed-meshheading:15680284-Iron,
pubmed-meshheading:15680284-Nitric Oxide,
pubmed-meshheading:15680284-Oxidation-Reduction,
pubmed-meshheading:15680284-Protein Structure, Secondary,
pubmed-meshheading:15680284-Quaternary Ammonium Compounds,
pubmed-meshheading:15680284-Ultraviolet Rays
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pubmed:year |
2005
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pubmed:articleTitle |
Direct electrochemistry of hemoglobin in dimethyldioctadecyl ammonium bromide film and its electrocatalysis to nitric oxide.
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pubmed:affiliation |
Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Hankou Road 22, Nanjing 210093, P.R. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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