Linked Life Data

15657938

Source:http://linkedlifedata.com/resource/pubmed/id/15657938

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-3-14
pubmed:abstractText
To gain insights into the role of purinergic receptors in oligodendrocyte development, we characterized the expression and functional activity of P2 receptors in cultured rat oligodendrocyte progenitors and investigated the effects of ATP and its breakdown products on the migration and proliferation of this immature glial cell population. Using Western blot analysis, we show that oligodendrocyte progenitors express several P2X (P2X(1,2,3,4,7)) and P2Y (P2Y(1,2,4)) receptors. Intracellular Ca(2+) recording by Fura-2 video imaging allowed to determine the rank potency order of the P2 agonists tested: ADPbetaS = ADP = Benzoyl ATP > ATP > ATPgammaS > UTP, alpha,beta-meATP ineffective. Based on the above findings, on pharmacological inhibition by the antagonists oxATP and MRS2179, and on the absence of alpha,betameATP-induced inward current in whole-cell recording, P2X(7) and P2Y(1) were identified as the main ionotropic and metabotropic P2 receptors active in OPs. As a functional correlate of these findings, we show that ATP and, among metabotropic agonists, ADP and the P2Y(1)-specific agonist ADPbetaS, but not UTP, induce oligodendrocyte progenitor migration. Moreover, ATP and ADP inhibited the proliferation of oligodendrocyte progenitors induced by platelet-derived growth factor, both in purified cultures and in cerebellar tissue slices. The effects of ATP and ADP on cell migration and proliferation were prevented by the P2Y(1) antagonist MRS2179. By confocal laser scanning microscopy, P2Y(1) receptors were localized in NG2-labeled oligodendrocyte progenitors in the developing rat brain. These data indicate that ATP and ADP may regulate oligodendrocyte progenitor functions by a mechanism that involves mainly activation of P2Y(1) receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0894-1491
pubmed:author
pubmed:copyrightInfo
(c) 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
132-44
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15657938-Adenosine Diphosphate, pubmed-meshheading:15657938-Adenosine Triphosphate, pubmed-meshheading:15657938-Animals, pubmed-meshheading:15657938-Animals, Newborn, pubmed-meshheading:15657938-Blotting, Western, pubmed-meshheading:15657938-Calcium Signaling, pubmed-meshheading:15657938-Cell Differentiation, pubmed-meshheading:15657938-Cell Movement, pubmed-meshheading:15657938-Cell Proliferation, pubmed-meshheading:15657938-Cell Survival, pubmed-meshheading:15657938-Cells, Cultured, pubmed-meshheading:15657938-Cerebellum, pubmed-meshheading:15657938-Chemotaxis, Leukocyte, pubmed-meshheading:15657938-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:15657938-Electrophysiology, pubmed-meshheading:15657938-Immunohistochemistry, pubmed-meshheading:15657938-Microscopy, Confocal, pubmed-meshheading:15657938-Oligodendroglia, pubmed-meshheading:15657938-Organ Culture Techniques, pubmed-meshheading:15657938-Purinergic P2 Receptor Agonists, pubmed-meshheading:15657938-Rats, pubmed-meshheading:15657938-Rats, Wistar, pubmed-meshheading:15657938-Receptors, Purinergic P2, pubmed-meshheading:15657938-Receptors, Purinergic P2Y1, pubmed-meshheading:15657938-Stem Cells
pubmed:year
2005
pubmed:articleTitle
Metabotropic P2 receptor activation regulates oligodendrocyte progenitor migration and development.
pubmed:affiliation
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy. agresti@iss.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't