Source:http://linkedlifedata.com/resource/pubmed/id/15644897
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2005-1-12
|
| pubmed:abstractText |
A total of 1500 environmental strains of Vibrio parahaemolyticus, isolated from the aquatic environment of Bangladesh, were screened for the presence of a major V. parahaemolyticus virulence factor, the thermostable direct haemolysin (tdh) gene, by the colony blot hybridization method using a digoxigenin-labeled tdh gene probe. Of 1500 strains, 5 carried the tdh sequence, which was further confirmed by PCR using primers specific for the tdh gene. Examination by PCR confirmed that the 5 strains were V. parahaemolyticus and lacked the thermostable direct haemolysin-related haemolysin (trh) gene, the alternative major virulence gene known to be absent in pandemic strains. All 5 strains gave positive Kanagawa phenomenon reaction with characteristic beta-haemolysis on Wagatsuma agar medium. Southern blot analysis of the HindIII-digested chromosomal DNA demonstrated, in all 5 strains, the presence of 2 tdh genes common to strains positive for Kanagawa phenomenon. However, the 5 strains were found to belong to 3 different serotypes (O3:K29, O4:K37, and O3:K6). The 2 with pandemic serotype O3:K6 gave positive results in group-specific PCR and ORF8 PCR assays, characteristics unique to the pandemic clone. Clonal variations among the 5 isolates were analyzed by comparing RAPD and ribotyping patterns. Results showed different patterns for the 3 serotypes, but the pattern was identical among the O3:K6 strains. This is the first report on the isolation of pandemic O3:K6 strains of V. parahaemolyticus from the aquatic environment of Bangladesh.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0008-4166
|
| pubmed:author |
pubmed-author:BakhtHabibul Bari MahmudHB,
pubmed-author:ColwellRita RRR,
pubmed-author:HuqAnwarA,
pubmed-author:IslamM SirajulMS,
pubmed-author:KhanSirajul IslamSI,
pubmed-author:MahmoodZahid HayatZH,
pubmed-author:NairG BalakrishGB,
pubmed-author:NishibuchiMitsuakiM,
pubmed-author:RahmanM ZiaurMZ,
pubmed-author:SackDavid ADA,
pubmed-author:SackR BradleyRB,
pubmed-author:SnowT LTL,
pubmed-author:TasminRizwanaR
|
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
827-34
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:15644897-Bacterial Toxins,
pubmed-meshheading:15644897-Bangladesh,
pubmed-meshheading:15644897-Blotting, Southern,
pubmed-meshheading:15644897-DNA, Bacterial,
pubmed-meshheading:15644897-DNA Fingerprinting,
pubmed-meshheading:15644897-Gene Dosage,
pubmed-meshheading:15644897-Genes, Bacterial,
pubmed-meshheading:15644897-Hemolysin Proteins,
pubmed-meshheading:15644897-Molecular Epidemiology,
pubmed-meshheading:15644897-Nucleic Acid Hybridization,
pubmed-meshheading:15644897-Open Reading Frames,
pubmed-meshheading:15644897-Polymerase Chain Reaction,
pubmed-meshheading:15644897-Random Amplified Polymorphic DNA Technique,
pubmed-meshheading:15644897-Ribotyping,
pubmed-meshheading:15644897-Serotyping,
pubmed-meshheading:15644897-Vibrio parahaemolyticus,
pubmed-meshheading:15644897-Water Microbiology
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Pandemic strains of O3:K6 Vibrio parahaemolyticus in the aquatic environment of Bangladesh.
|
| pubmed:affiliation |
International Centre for Diarrhoeal Disease Research, Bangladesh, GPO 128, Dhaka 1000, Bangladesh. sislam@icddrb.org
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|