Linked Life Data

1556108

Source:http://linkedlifedata.com/resource/pubmed/id/1556108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1992-5-4
pubmed:databankReference
pubmed:abstractText
Human selenium-dependent glutathione peroxidase (hGPx1) (EC 1.11.1.9) is thought to be involved in many critical cellular functions as a result of its role in glutathione-mediated reduction of toxic peroxides, and it is implicated as a mechanism of resistance against oxygen free radicals. Previous studies have demonstrated that the gene encoding hGPx1 (hgpx1) is more highly expressed in multidrug-resistant AdrR MCF-7 human breast cancer cells than in the parental WT MCF-7 cell line. In order to further study the transcriptional regulation of hgpx1, we have cloned the genomic hgpx1 gene and determined its nucleotide sequence. The 2550-base pair (bp) 5'-flanking sequence of hgpx1 contained the terminal 511 bp of the 3' end of a previously reported rhoH12 cDNA (Yeramian, P., Chardin, P., Madaule, P., and Tavitian, A. (1987) Nucleic Acids Res. 15, 1989), a ras-related oncogene. Further downstream from rhoH12, but before the start of transcription of hgpx1, RNase protection analysis revealed a transcribed sequence of at least 270 bp which we have called mid. RNA transcripts homologous to both rhoH12 (1.8 and 1.5 kilobase pairs (kb)) and mid (1.8 kb) are also more highly expressed in AdrR MCF-7 cells than in WT MCF-7 cells. We screened an AdrR MCF-7 cDNA library with the mid sequence and isolated a partial cDNA clone which contains both mid and rhoH12 sequences and is colinear with the genomic sequence which extends from 10 bp 3' to the rhoH12 stop codon to 810 bp 5' to the start of transcription of hgpx1. The start of transcription of hgpx1 in AdrR MCF-7 cells was determined by primer extension analysis. The promoter and 2 kb of the 5'-flanking sequence of hgpx1 was fused to the bacterial chloramphenicol acetyltransferase gene (hGPx1-CAT1). Analysis of deletion constructs of hGPx1-CAT1 revealed three possible cis-acting regulatory regions. The transcriptional regulation of hgpx1 was examined using the hGPx1-CAT hybrid genes and nuclear run-on studies. We found no evidence that increased mRNA transcript formation could account for different levels of hgpx1 RNA either in different breast cancer cell lines or in response to selenium.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
267
pubmed:geneSymbol
hgpx1, mid, rhoH12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5949-58
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:1556108-Amino Acid Sequence, pubmed-meshheading:1556108-Base Sequence, pubmed-meshheading:1556108-Blotting, Northern, pubmed-meshheading:1556108-Blotting, Southern, pubmed-meshheading:1556108-Cell Line, pubmed-meshheading:1556108-Cloning, Molecular, pubmed-meshheading:1556108-Cytosol, pubmed-meshheading:1556108-DNA, Neoplasm, pubmed-meshheading:1556108-DNA Probes, pubmed-meshheading:1556108-Genes, pubmed-meshheading:1556108-Genes, Regulator, pubmed-meshheading:1556108-Genomic Library, pubmed-meshheading:1556108-Glutathione Peroxidase, pubmed-meshheading:1556108-Humans, pubmed-meshheading:1556108-Leukocytes, pubmed-meshheading:1556108-Male, pubmed-meshheading:1556108-Molecular Sequence Data, pubmed-meshheading:1556108-Oligodeoxyribonucleotides, pubmed-meshheading:1556108-Restriction Mapping, pubmed-meshheading:1556108-Selenium
pubmed:year
1992
pubmed:articleTitle
Structure and function of the 5'-flanking sequence of the human cytosolic selenium-dependent glutathione peroxidase gene (hgpx1).
pubmed:affiliation
Medicine Branch, National Cancer Institute, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article